The Effect Of Raloxifene, A Selective Estrogen Receptor Modulator, On Blood Pressure, Lipid, Fibrinogen And Estrogen Receptor In Ovariectomized Rats

Objective: To investigate the effect of raloxifene, a selective estrogen receptor modulator, on blood pressure, lipid, fibrinogen and estrogen receptor in ovariectomized rats.Methods: Forty twelve-week-old female Sprague-Dawley rats were randomly divided into five groups: (1)ovariectomy(OVX); (2)sham operation(C); (3) ovariectomy + estrogen(1 mg/kg·d , E); (4) ovariectomy + raloxifene(1 mg/kg·d , Rs); (5) ovariectomy + raloxifene(3 mg/kg·d , Rl). Serum estradiol, blood pressure, serum lipid and fibrinogen were measured after three months treatment. Took rats'heart, thorax aorta and kidney as samples and compared the expression levels of estrogen receptor and its mRNA in these samples among all groups by immunohistochemistry and RT-PCR.Results:(1) Serum estradiol concentration was significantly lower in ovariectomized rats than that in sham operated animals. Serum estradiol was increased after the treatment of raloxifene(OVX:36.69±6.91 vs C:248.32±32.51 vs E:240.89±29.43 vs Rs:239.56±25.73 vs Rl:243.48±27.62 pg/ml,P﹤0.01).(2) The systolic blood pressure(SBP) was significantly higher in ovariectomized rats than that of other groups, SBP was decreased after the treatment of raloxifene(before treatment:OVX:103.67±9.98 vs C:102.74±12.20 vs E:104.36±16.86 vs Rs:102.36±15.76 vs Rl:104.37±11.22,P>0.05;after treatment: OVX: 128.46±15.78 vs C:104.48±15.03 vs E:106.83±13.52 vs Rs:109.55±11.23 vs Rl: 107.57±17.56 mm Hg,P﹤0.01).(3) Serum cholesterol(CHOL) and low-density lipoprotein(LDL-C) were...