Study On The Targeted Expression Of Suicide Gene Induced By Radiation In Nasopharyngeal Carcinoma Cell

Objective: TO clone the promoter of Egr-1 gene of human and construct recombinant eukaryotic expression plasmid pcDNA3.1(+)Egr-1-CD , trans-fecting into human nasopharyngeal carcinoma cell line by LipofectamineTM , induct the targeted expression of CD/5-Fc Suicide gene system in positive clone nasopharyngeal carcinoma cell line by ionizing radiation,observe the relation between ionizing radiation dose effect and gene expression .Establish tumor gene-radiotherapy system.The tumor cells will be killed simultaneously by gene and irradiation ,lower the radiation dose and decrease the damage of normal tissue.Methods:Design a pair of primers to clone human Egr-1 promoter basing human Egr-1 gene sequence in Genbank .Using PCR, enzyme restriction, ligation and other molecular technology to construct pcDNA3.1(+)Egr-1-CD recombinant expression plasmid,and transfecting into human nasopharyngeal carcinoma cell line by Lipofectamine^TM and were cultured in G418 medium, it was proved that the transfecting was successful by RT-PCR. observe the relation between ionizing radiation dose effect and gene expression in different irradition dose . Establish tumor gene-radiotherapy system. Observe the kill effect of different irradition dose in positive clone nasopharyngeal carcinoma cell line through the teamed experiment in vitro.Cell livability was...