The Effects Of Berberine Chloride On Experimental Murine Colitis Induced By Dextran Sulfate Sodium

Objects: To explore the protective effects and the possible mechanism of berberine chloride(BER) on experimental colitis induced by dextran sulfate sodium in mice. Methods: 1 , Animal model: An experimental colitis animal model was established by administrating ad libitum 4% dextran sulfate sodium in distilled water for 7d. 2,Disease activity index: The daily measurement of weight, stool frequency, and guaiac testing was obtained to assess the clinic symptom of the experimental mice. The disease activity index was combined scores of weight loss, stool consistency and beeding divided by 3. 3,Biochemical examinations: To determine the activities of MPO, SOD and the concentration of MDA , according to the methods of the presentations of these kits. 4,Pathomorphology and immunohistochemistry: Histopathological slices were stained routinely with HE and the pathological changes in colon mucosa were detected under the microscope. Immunohistochemistry was performed using a SP technique. The content of experiment: 1,To establish the experimental murine colitis model. 2,To investigate whether BER and TAL exhibit any protective effect against the colon mucosa damage induced by 4% dextran sulfate sodium. 3,To explore the possible mechanism of the protective actions of BER and TAL on the experimental colitis induced by 4% dextran sulfate sodium. Results: 4%DSS induced experimental colitis in BALB/C mice with resultant weight loss, accompanied by the presence of occult blood and even rectal bleeding, and loose stools; epithelial ulceration, crypt shortening or even loss, and infiltration of neutrophils, lymphocytes, and macrophages in colon mucous membrane. Treatment of SASP in DSS-induced mice exhibited significantly lower level of DAI, MPO activity, MDA content, and the expressions of NF-κBp65 and ICAM-1 proteins, while higher level of SOD, compared with vehicle-treated mice. BER and TAL had the similar effects to SASP in DSS-induced mice. Conclusion: BER and TAL have protective effects on intestinal inflammation in DSS-induced colitis. The mechanisms possibly contribute to their antioxidant function and anti-inflammatory activity associated with the effects of inhibiting the NF-κB activation and the inflammatory cells infiltration.