Relationship Between Paraoxonase 1,Paraoxonase 2 And Apoliplprotein E Gene Polymorphism And Type 2 Diabetic Nephropathy

【Objectives】1 To study the effects of the disorders of serum glucose,lipid metabolism and the enhancement of lipid peroxidation on type 2 diabetic nephropathy. 2. To investigate the singular and combined effects of the paraoxonase-1 R192Q,paraoxonase-2 S311C and Apoliplprotein E polymorphisms in relation to type 2 diabetic nephropathy. 3.To study possible mechanisms of the relationship between the genovariations and diabetic nephropathy.【Methods】The allele frequency and the genotype distribution of the polymorphisms of PON1 gene,PON2 gene and ApoE gene in the group of 105 normal subjects and 210 type 2 diabetes were detected by means of polymerase chain reaction-restriction fragment-length(PCR-RFLP). According to the albumin-to-creatinine ratio(ACR), the group of type 2 diabetes was divided to diabetic nephropathy positive(DN+) group and diabetic nephropathy negative(DN-) group. The serum concentration of malonaldehyde (MDA) was qualified by the TBA assay. At the same time the clinical dates such as FBG,HbA1C,TC,TG,LDL,BP,BMI were obtained【Results】...