Expressions Of Midkine,Skp2 In Colorectal Cancer And Their Clinical Significance

Background: Colorectal cancer is one of the commonest malignant tumors in China and its morbidity is still increasing . Although the operation-based comprehensive therapies have greatly improved the prognosis of colorectal cancer patients in recent years, the postoperative 5-year survival rate, unfortunately, is still about 50%. Therefore, further exploring the alterations of key genes related to development and progression of CRC will be considered useful.Aims: In this study, We sought to investigate the expression of Midkine mRNA, Skp2 mRNA and Midkine protein, Skp2 protein, MVD, p27^kip1 protein in colorectal cancer, and to identify the clinical significance of Midkine, Skp2 and its relationship with the expression of p27^kip1 and MVD in colorectal cancer, to provide theoretical basis for early diagnosis and therapy of colorectal cancer.Materials and Methods: (1) Tissue specimens used for this study were obtained from 101 patients with colorectal cancer or adenoma, which were resected surgically or endoscopically at Hospital from Jan. 2000 to Oct. 2004. All the samples including 20 normal colorectal mucosae, 18 adenomas and 83 cancers were fixed in 4% Polyoxymethylene(0. 1%DEPC) and embedded in paraffin. (2) The expression of Midkine mRNA, Skp2 mRNA was detected by in situ hybridization and the...