Font Size: a A A

Effects Of Seawater Immersion On Wound Repair In Scalded Rats And Its Mechanism

Posted on:2007-03-23Degree:MasterType:Thesis
Country:ChinaCandidate:Z F FanFull Text:PDF
GTID:2144360185988446Subject:Burn surgery
Abstract/Summary:PDF Full Text Request
ObjectivePeople who operate or battle in the sea are easy to be wounded by all kinds of injuries. The wound is often contaminated by seawater. It will do harm to the body and affect wound healing. Up to now it remains little known the effects of seawater immersion on wound repair and its mechanism. In this study we used a model of 10% TBSA deep second degree scalded rats to investigate the effects of seawater immersion on wound repair and its mechanism from the aspects of pathological change in wound, flora immigration and changes of serum inflammation mediators, to provide reference for clinical treatment of the burn with seawater immersion. Methods1, Animal model: 192 male Wistar rats weighted 200-250 g were randomized into scalded control group (group A), seawater immersion 1h (group B), 2h (group C) and 4h (group D) with 48 rats each group. One day before scalded, hair on rats' back was depilated by 80g/L sodium sulfide. 25g/L sodium amytal was injected into the abdominal cavity for anaesthesia with the dosage of 35 mg/kg before scalded, then 10% TBSA deep second degree scald was made by immersing rats in 80℃ hot water for 15s. Except group A, hands and feet of the rats were tied up and body below the upper limbs was immersed in seawater after scalded for 1, 2,4 h separately.2, Test index and methods: Wound infection status and inflammation around the wound were observed by eyes at 1, 3, 5, 7 d after scalded. Histopathological changes...
Keywords/Search Tags:Burn, Seawater, Tumor necrosis factor-a, Interleukin-6, Infection, Wound healing
PDF Full Text Request
Related items