In Vitro Studies On The Antitumor Activity Of HC-5, A Component Extracted From Microorganisms

The in vitro antitumor activity of HC-5, a component extracted from microorganisms was studied in this dissertation.SRB results showed that HC-5 inhibited the proliferation of A2780( the Human Ovarian Cancer Cell Line ) K562( the Chronic Myeloid Leukemia Cell Line ).. HCT-15( the Human Colorectal Adenocarcinoma Cell Line ) Hela( the Human Cervix Epithelial Cell Line) and A549( the Lung Alveolar Epithelial Cell Line ) cell lines in a dosage dependent manner. The inhibition rate increased dramatically when the dosage was over 1MM( including 1μM). The IC₅₀ Value in these cell lines was respectively : A2780 (1.376± 0. 385μM) K562 (8. 409±4. 735μM) HCT-15 (9. 392±2.248μM) Hela (13. 684 + 2. 032μM) A549 (15. 431 + 5. 567μM) .FCM results showed that A2780 cells treated with HC-5 were remarkably arrested at G₀/G₁ phase. When A2780 cells were treated with 0.1 and 1 μ M HC-5 for 24h, the percentages of G₀/G₁ phase cell number were 74.933%( P<0.05) and 86.267%( P<0.001) respectively compared with 52.267% of control group. On the other hand, when A2780 cells were treated with 1 μM HC-5 for 6, 12 18 and 24h , the percentages of G₀/G₁ phase cell...