The Inhibition On Ovarian Cancer Cell By Microorganism Fermentation Product LMC

This paper focused on the study of the anticancer activity of LMC in vitro. LMC is a compound extracted from the fermentation products of microorganisms.At first, we detected the LMC on the proliferation activity of A2780 by SRB assay. The IC50 Value of LMC for A2780 was: 1.376±0.385μM. The proliferation of A2780( one of the Human Ovarian Cancer Cell Lines) was inhibited by LMC in a dosage dependent manner, and this inhibition effect was increased obviously at higher dosages over 1μM( including 1μM ) .We detected the LMC effect on A2780 cell line by FCM, it was found that the cell percentage of sub-G₀/G₁ phase was remain basically unchanged and the cell percentage of G₀/G₁ phase was significantly increased. The changes of cell percentage were the time-dependent manner. The results indicated that the inhibition of LMC on cell proliferation was not caused by apoptosis, but should be a role G₀/G₁ phase arrest.We also tested the LMC effect on A2780/cDDP cell line by FCM. The results showed that LMC had a strong inhibition effect on cell line A2780/cDDP, and the inhibition mechanism was also the G₀/G₁ phase arrest. Moreover, the inhibition rates of LMC were similar on both cell lines A2780 and A2780/cDDP.Western blotting assay results showed that, after treatment with 1μM of LMC, P21 and P27 were found to be increased noticeably, Cyclin D was decreased significantly, Cyclin E also decreasd lightly. All of the above changes were time-dependent. In case of other related proteins such as P53,CDK4 and Rb, no changes were observed. These results indicated that LMC probably cause G₀/G₁ phase arrest through the inhibition of cell proliferation.