Studies On The Alkloids Of Silkworm Feces

Silkworm feces is the excrement of silkworm, which is one of the Chinese traditional medicine, after the silkworm nibbles the mulberry leaves. The dried silkworm feces is presented a small columned granule of massiness and uniformity which is black or blackish green. Due to its remarkable effect of the cure of the diabetes,antivirus and anticancer, it arouses the attention of many researchers in the world. The paper is mainly about the chemical constituents of the silkworm feces especially the alkaloids, and the general situation of the study on the silkworm has been reviewed in this thesis.The systematic preliminary tests have been done by the methods of the tube-test. It was found that it contains flavonoids, steroids, terpenoids, phenols, lact ones, coumarins, alkaloids, carbohydrates, amino acids, proteins; probably contains tannides, saponins, thraquinones , volatile oils; contanis no cardiac glycosides.The silkworm feces was extracted by ultrasonic with deionized water and filtered. the extraction solution was adsorbed by 732# cation exchange resin, then eluted with deionized water until the color of the eluate became colorless, after that, the solution of 1 mol/L hydrochloric acid was used to elut the resin until the eluate can't be colored with ninhydrin. Thus, the concentrated eluant extraction mainly contained alkaloids. The extraction was divided into two parts, was dealed with in different methods: the first part was extracted by solvent with different polarity, then separated with colum chromatography of silica gel; the second part was filtered through macroporous resin LSA-30, and the components'structure were decorated with 2,4-dinitrofluorobenzene, then seperated with colum chromatography of silica gel.Finally, nine compounds were isolated from the water extraction of silkworm feces; two compounds was isolated from structure qualification. Based on their spectral data(IR,ESI MS,1H NMR,13C NMR),three of them have been confirmed,which are 1-deoxynojirimycin, coumarins,alaine. The compound 7and 8 may both are 1-deoxynojirimycin, only with differences in their configurations.The antibacterial activity of different extractions were tested with dish diffusion of three different concentration. The result showed that ethyl acetate extract had the strongest...