The Effect Of Antisense Sequences Of Epidermal Growth Factor Rceptor On Proliferation Of Vascular Smooth Muscle Cells Activated By Ang Ⅱ

AIM angiotensin II (Ang II) , the major bioactive peptide of the rennin-angiotensin, play a fundmental role ,not only in controlling cardiovascular and renal hemoeostasis but also contributing to various cardiovascular disease such as hypertension ,atherosclerosis and heart failure .The researchs have demonstrate that EGFR has a significant function in signaling. Ang II transactivate EGFR via AT1 receptor and EGFR itself is one of tyrosine kinases. Activated EGFR can activate mitogen activation protein kinases (MAPKs) pathway which lead to growth and proliferation of cells. This experiment had use EGFR oligodeoxynucleotides(ODNs) as a selective inhibitor to inhibit the expression of EGFR mRNA and protein in vascular smooth muscle cells (VSMCs) and was to evaluate the effects of EGFR ODNs on proliferation of VSMCs activated by Ang II. It is to investigate the effect of EGFR on proliferation of VSMCs activated by Ang II in the level of molecular biology.METHODS Sprague-Dawley rat VSMCs were transfected with antisense sequences of EGFR and Stimulated by Ang II. EGFR mRNA and protein in VSMCs were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western-Bloting. Proliferation of VSMCs was detected by ~3H-Tdr incorporation. RESULTS The expression of EGFR mRNA and protein in VSMCs...