Detection And Clinical Application Quantification Of Bcr/abl Transcripts In Patients With Chronic Myeloid Leukemia By Real-time Quantitative Reverse Transcriptase Polymerase Chain Reaction

Objective: Establish a method to investigate the effect of real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) approach in chronic myeloid leukemia (CML) for detecting the minimal residual disease (MRD) or monitoring the treatment response and predicting the prognosis.Methods: Set up stablely experimental condition use K562 cell line. Expression of bcr/ abl gene was measured with RQ-PCR in 34 cases of CML at different stages. Tirty-four CML patients, 20 males and 14 females, aged 35 (17 approximately 69), 17 of which were in the incipient chronic phase, 9 in the incipient phase, 1 in the accelerated phase, and 4 in the rapidly progressing phase, 3 after Allo-BMT. To assess the relationship between expression of bcr/ abl mRNA and the diagnosis as well as prognosis of CML, and the significance in the detection of MRD.Results: Experiment revealed that the sensibility of RQ-PCR can reach 10~1 copies. No bcr/ abl gene was revealed in the controls, while in the chronic myelogenous leukemia (CML) group the positivLe rate was 96. 77 %( 30/31), three patients were negative after Allo-BMT. With an average CT numerus bcr/ abl gene expression of 37.14 in the chronic phase, while in those others the average expression was 26. 43. The difference between two groups...