| Objective To explore the Functional recovery of the spinal cord through transplant CD34~+ cells from bone marrow into the completely transected rat modal. Methods To culture the bone marrow cells from transgenic GFP mouse in vitro. After identified by anti-CD34 monoclonal antibodies, we transferred the cells into the caudal of transaction. To survey the motor functional recovery of hind limbs. Respectively at the 24h, 48h,1w, 2w, 4w and 8w, to detect the locomotor functions of the rats by BBB score, then infused paraformaldehyde into left ventrical of the rat modal. Through sank and frozen, the spinal cord was sectioned at lOum thickness. To observe the green fluorescence cells through the fluorescence microscope. To dentify CD34~+ cells by immunohistochemistry staining. Result I. Suspension cells of isolated group grew well within 7 days. The cells had different shapes and numbers during the cultural course. On the 7th day, the rates were CD34~+ cells >76%. 2. Diffusing or accumulating distribution of green fluore- scence cells could be found in gray matter of the rostral part and caudal part around the transected sites. Scattered CD34~+ cells can be stained by immunohistochemistry. 3. In comparison with the control group, the more... |
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