The Experimental Study Of Mesenchymal Stem Cells Combined With Inbred Line Small-ear Pig Bone On Repairing Segmental Bone Defects

Objective To explore the feasibility of the mesenchymal stem cells combined with inbred line small-ear pig bone on repairing segmental segmental bone defects. Methods MSCs were cultured with inbred line small-ear cancellous bone of Ban Na pigs . A 1.5 centimeter segmental defect was created in the mid-upper part of the radial shaft of adult rabbit. The defects were implanted into composited xenogeneic bone in experimental group and xenogeneic bone alone in control group , the defects were not filled with anything in blank group . The repair capability of the defects was assessed by scan electron microscope(SEM) before operation and physical , histology, X-ray , transmission electron microscope (TEM), SPECT and bone mineral density examinations 4 , 8 and 12 weeks after operation . Results 12 weeks after operation, the defects were repaired completely and its bone mineral density is near to the normal bone in experimental group , the defects were repaired slowly and the quantity of new bone fonnation is less in control group , the defects of blank group were not repaired.Conclusion MSCs composited with inbred line small-ear pig bone on repairing segmental bone defects is obviously superior to the single xenogeneic bone in quantity and quality of new bone formation.