| Objectives To study the survival, growth and function restoration of fetal ovary allotransplation and evaluation the effect on fetal ovaries cryopreservation with ultrarapid cooling.Methods 18～20d rat fetal ovaries were obtained. Ultrarapid cooling was the protocol for cryopreservation of fetal ovaries: cryoprotectant medium involved 40% ethylene glycol+0.35 M sucrose+10% egg yolk. Fetal ovaries were loaded in 0.25ml straws and directly plunged into -196℃ liquid nitrogen. After 4 weeks, cryopreserved fetal ovaries were fast thawed. The fresh and frozen-thawed ovaries were ectopic allotransplanted subcutaneously to adult female SD rats had been crastrated for 4 weeks. Vaginal smears were taken daily after transplantation to observe the restoration of oestrous cycles. On days 0,21,35,49,63 after transplantation, blood was obtained from rats tail and serum estrodiol (E2) and progesterone (P) levels were measured by chemiluminescence analysis. On 63 days after transplantion, all rats were killed. Wet weight of uterus, histological morphology of endometrium and ovaries were compared among four groups.Results 52.18% of the recipients in fresh group and 38.01% in frozen-thawed group resulted in restoration of regular oestrous cyclic activaty, this value was no statistically significant difference between two groups (P>0.05). The estrous cycle recovered on 24.25 ± 1.91 days and 29.5 ±11.41 days after ovaries transplantation.
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