| OBJECTIVE The objective of this thesis is to construct a research platform on anti-hyperuricemia complex prescription for pharmacodynamics and mechanism of action. In addition, the platform is used to investigate modified Si Miao Powder ("whole prescription" for short) and its effective fractions ("group A" for short) .METHODS We first make use of the biological metabolism-enzymology to measure the index of rats' blood, urine, liver, kidney, joint cavity and so on. Several methods are adopted to observe the anti-inflammation, abirritation and lowering uric acid effect of whole prescription and group A. These methods include the mouse' pain reaction to chemical stimulation, the mouse' ear swelling induced by xylene, rat swelling models induced by MSU, mouse and rats' hyperlithuria models. Finally, we Compare the mechanism of action of whole prescription and group A. RESULTS With the same dosage, whole prescription and group A could lower serum uric acid on acute hyperuricemia mice and rats, inhibit inflammation reaction caused by MSU and xylene, and relieve pain injected by HAC . In the continuity hyperuricemia model which is replicated by uricase inhibitor, serum uric acid, urea nitrogen, and creatinine in rats were all kept on a high level. Furthermore, urine volume, uric acid concentration in urine, ken homogenate and synovial fluid were much higher than those of the corresponding blank group (P<0. 01) . Group A could lower the uric acid concentration in urine, ken homogenate and synovial fluid obviously (P<0. 01) , and whole prescription could also reduce the uric acid concentration in urine and synovial fluid (P<0. 01) . The activity of serum xanthin oxydase of the model group is more powerful than that of the blank group (P<0. 05) , which could also be depressed by group A remarkably (P<0. 01) . For another hyperuricemia model which is replicated by eccrisis uric acid inhibitor, concentration in serum uric acid could...
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