Effect Of Tumor Necrosis Factor-α On Expression Of Brain AQP-4 Of Mouse With Fulminant Hepatic Failure

PrefaceThe prognosis of severe hepatitis is serious,and its case fatality is high. The hepatic encephalopathy (HE) is one of the severe complications, and the brain edema happen on about 80% HE patients which can directly lead to death, but the pathogenesis of brain edema is not clear. Recently some research suggests that aquaporin is correlated with the pathophysiologic mechanisms of brain edema. In normal brain AQP -4 water channel is a important aquaporin,In this research we want to find the effect of tumor necrosis factor - αon expression of brain AQP - 4 of mouse with fulminant hepatic failure ( FHF) and its significance.Materials and method1. The animals and make model(1) Animals :The condition of experimental animal is 60 Balb/c mouse is 6 -8 weeks old,and their body weight are 18 -22g. And make 8 groups in random:1. NS control group;2. LPS control group;3. GalN control group; 4. TNFα control group;5. LPS FHF group(LPS + GalN) 2,6,9h;6. TNFα FHF group: (TNFα + GalN) 2,6,9h; 7. TNFα antibody group (TNFa - IgG antibody + TNFα + GalN) ;8. TNFα - R1 antibody group: (TNFα - R1 antibody + TNFα + GalN).(2) Make FHF model :LPS,GalN and TNFαis peritoneal injection to make the FHF model. TNFα - IgG antibody and TNFα - R1 antibody is injected into caudal vein. We took the brain and the liver of model mouse at the laying time.