Foundamental Study On The Estrogenlike Activity Of Samia Cynthia Ricini On Reproductive System In Mice

To develop an ideal medicine resource for HRT and promote the value of Samia cynthia ricini in use, we prepared the extract of female moths Samia cynthia ricini (EEFM). And then, we used ovariectomized mature female mice and premature female mice as models to simulate the menopausal symptom, and evaluated the optimal site of EEFM in this report on two-direction biological activities such as the coefficients of the uterus and immune organs, keratinization rates of vagina epithelia and peroxidase (POD) activities, et al. The optimal site of EEFM was also analysed by gas chromatography-mass spectrometry (GC-MS). The result showed that EEFM had remarkable effect on the coefficients of the uterus and immune organs, keratinization rates of vagina epithelia and POD activities. It was found that all kinds of ethanol extractions of female moths had significant estrogenic effect on subject mice at the dose of 800mg/kg by testing the coefficient of uterus, immune organs, and the keratinization rates of vagina epithelia. All extractions had no negative effect on immune organs while diethylstilbestrol obviously decreased the weight of thymus. The comparison among all the ethanol extractions revealed that the extraction with 80% ethanol (EEFM80) has the most remarkable estrogenic effect of all the ethanol extractions. The estrogenic activity of the 80% ethanol extraction (EEFM80) compared with other common clinical estrogens such as estradiol valerate, diethylstilbestrol, CEE, and so on were studied. The result indicated that the estrogenic activity of EEFM80 at 800mg/kg.d was close to those of estradiol and CEE at 0.085mg/kg.d and that EEFM80 clearly increased the thymus coefficients of subject mice. According to this research, it was concluded that EEFM80 had significant estrogenic effect on subject mice and showed no harm...