Antimutagenicity Of Micrometer Powder Of Selenium-Enriched Green Tea

Green tea has the potential of antimutagenicity, the selenium of plant source is also provided with the antimutagenic efficacy. However, there are few reports about antimutagenic ability of micrometer powder of selenium-enriched green tea (MPSGT). In this study, green tea was used as selenium carrier, according to invent potent (ZL 03112724. X): a kind of production regulator and direction about Se-enriched tea leaves, Se-enriched tea leaves were processed, meanwhile, regular green tea was prepared. The mice were fed with selenium-deficient basal diet according to AIN93M diet guidelines. Se-enriched green tea and regular green tea were made into MPSGT and micrometer powder of regular green tea (MPRGT) with the diameter of 10-25 urn using the machine. MPSGT was applied in conducting acute toxicity test, and on the base of acute toxicity test, Mice Bone Marrow Cell Micromucleus Test and Mice Testicle Chromosome Aberration (CA) Test were conducted to evaluate the antimutagenic efficacy. At the end of animal trials, the liver and blood of mice were sampled for the determination of selenium content, meanwhile, GSH-Px and SOD activities were also determined in serum and liver.The results are shown as follows: The LD₅₀ value of MPSGT was more than 10 g/kg, therefore, MPSGT was negligible risk of toxicity according to the evaluation criterion of GB 15193.3-1994.In mutagenic test, the frequency of micronucleated polychromatic erythrocytes (MNPCEs) was significantly lower in any of the treated groups compared to distilled water control. The incidence of micronuclei in polychromatic erythrocytes (PCEs) in groups treated with MPSGT was 0.9%-1.4‰, which was less than 6.2‰ in distilled water control. This indicated MSGTP had no potential of micronuclei induction. In CA test, MPSGT induced lower incidence of CA at any Se concentration tested in comparison with distilled water control, other treatments were negative for CA as well. Thus, MPSGT was negative for induction of MN and CA. On the contrary, MPSGT exhibited the activity of inhibiting the spontaneous MN frequency and spontaneous CA incidence. These results confirm that MPSGT posed no genotoxic risk and suppressed spontaneous mutation at the dose level in...