Rapid Diagnosis Of Severe Acute Pancreatitis Early Complicated With Bacterial Infection By Polymerase Chain Reaction

Objective: To explore the diagnostic value of 16s rRNA gene based bacteria polymerase chain reaction(PCR) method on diagnosing severe acute pancreatitis early complicated with bacterial infection.Method: The peripancreatic fluid from patients (13 females and 4 males,aged between 17 and 79) with severe acute pancreatitis were obtained by ultrasound-guided percutaneous fine needle aspiration (FNA) , the PCR assay was used to detect bacteria and compared with convention culture.Results: 45 samples from 17 severe acute pancreatitis cases were culture with 38 samples (10 cases)being bacteria positive (positive detection rate 84.4% )while in the PCR detection, 36 samples were (9 cases) positive (positive detection rate 80%) , of 17 samples was from FNA,of 28 samples from operation. The sensitivity of PCR ,culture to diagnose pancreatic infection was 90% (9/10) ,100% (10/10), respectively. Their specificity was 100%(7/7). The time for obtaining the results was five hours, three days.10 pantients were diagnosed as pancreatic infection and operated;4 pantients were diagnosed as non-pancreatic infection and cured by nonsurgity therapy;2 pantients were died.Conclusion: 1. Ultrasound-guided percutaneous fine needle aspiration was safe and feasible; 2.The PCR method was rapid,accurate way in the diagnosis of severe acute pancreatitis early complicated with bacterial infection and can offer faithful proof for operation.