Changes Of Bacterial Translocation And Intestinal Flora Composition In Rats With Severe Acute Pancreatitis Of Different Course

Objective:This paper is to study changes of intestinal flora and bacterial translocation in rats with severe acute pancreatitis(SAP).Methods:The healthy SD rats were randomly divided into Control group(n=10)and SAP group(n=30).The SAP group was divided into 6,12 and 24 h subgroups,with 10 rats in each subgroups.The model of severe acute pancreatitis of rats was induced by the method of injecting adversely 5% sodium taurocholate into the common billiary-pancreatic duct.The rats were killed of 6,12 and 24 h respectively.The serum amylase and endotoxin level were detected.Bacterial translocation rate in abdominal cavity and viscera was detected by bacterial culture,and positive colonies were identified by mass spectrometry.16 S r DNA and real-time fluorescence quantitative polymerase chain reaction were used to quantitative analysis of enteric bacteria.Pathological changes of pancreas and intestinal mucosa were observed in rats of 12 h subgroup.Results : The serum amylase in the SAP group was significantly higher than in the control group and more than three times(Control group =2086 ± 686 U/L,6h=8707±1672 U/L,12h=9012±1505 U/L,24h=8797±1298 U/L,P<0.01).The pancreatic tissue and small intestinal mucosa of 12 h subgroup had obvious pathological damage.As the course of the disease prolonged,the intestinal Escherichia coli in the SAP Group continued to proliferate(Control group =4.53±1.26,6h=10.07±2.57,12h=16.18±3.51,24h=19.45±4.70,P<0.01),the lactobacillus continues to decrease(Control group =23.12±4.46,6h=11.43±2.68,12h=5.20±2.07,24h=2.02±0.94,P<0.01),the bifidobacterium also continues to decrease(Control group =23.30±6.00,6h=17.61±3.94,12h=13.21±2.84,24h=9.49±3.41,P<0.01),and the positive rate of serum endotoxin test,the mesenteric lymph node and the bacterial translocation rate of abdominal viscera increased(serum endotoxin : Control group=0%,6h=30%,12h=50%,24h=60%;liver:Control group =0%,6h=20%,12h=50%,24h=70%;pancreas:Control group =0%,6h=30%,12h=40%,24h=60%;mesenteric lymph nodes:Control group =0%,6h=40%,12h=70%,24h=80%;P<0.01)Conclusions: In rats with severe acute pancreatitis,the intestinal mucosal barrier is damaged,the intestinal flora is disturbed,the proportion of aerobic bacteria and anaerobic bacteria in the intestinal cavity is inverted.As the course of the disease is prolonged,the pathogenic bacteria of Escherichia coli are increasing,and the anaerobic probiotics,such as Lactobacillus and Bifidobacterium,are constantly decreasing,causing intestinal bacteria and endotoxin.There is a certain relationship between displacement and SAP and concurrent pancreas and systemic infection.