Experimental Research On The Effects Of Medicined Diet On Immune Dysfunction In Severe Burned Rats

Objective:1. To observe the change of immunity function of severely burned rats;2. To observe the opsonization of medicined diet on immune dysfunction in severely burned rats;3. To investigate the possible mechanism of the opsonization of immune dysfunction of severely burned rats and the available methods to improve immunological function after burned.Methods:To design a kind of medicined diet. According to the making methods of medicined diet to decoct traditional Chinese drug into the medicinal broth, and then conserve them at 4°C after filtering the broth and getting rid of residual drug. There are 100 healthy Wistar rats with 50% female and 50% male wighting 200±20g were divided into the burned with lavaging medicined diet group(group A,n=30), the burned with lavaging bouillon group(group B,n=30) , the convention breeding group(group C,n=30) and the normal control group(group D,n=10)randomly. The rats were depilated with Na₂S alcohol solution on the back after 7 days of adaptability feeding. They were anesthetized with intraperitoneal administrating 5% ketamine hydrochloride (2ml/kg) after 12 hours' fasting, and then they are in prone position after satisfied anesthesia. To calculate 30%TBSA(total body surface area) on the basis of the formula S= K·W^2/3 (K=0.091,S=TBS cm²,W=Wight g) and adjust the burner cup of the burning application. The rats were burned on the back about 30%TBSA III degree through pathological verification and the rats of the group D were fake...