Screening Of AZF Microdeletion On Y Chromosome In The Patients With Azoospermia Or Severe Oligozoospermia | Posted on:2007-07-19 | Degree:Master | Type:Thesis | Country:China | Candidate:J Z Huang | Full Text:PDF | GTID:2144360212456591 | Subject:Obstetrics and gynecology | Abstract/Summary: | PDF Full Text Request | Objective: To assess the relationships between severe oligozoospermica or azoospermia and microdeletions on Y chromosome ; to set up the method for screening of microdeletions on Y chromosome ; to determin microdeletion loci and the characteristic of Y chromosome azoospermia factor(AZF) associated with jangxi azoospermia or severe oligozoospermia ; to approach the relationships between genotype and phenotype or gonadal hormone and provide some scientific theory basis for reasonable clinic therapy.Methods: we analysed the frequency of microdeletion on Y chromoome by 6 primes from three locus of AZF regions(AZFa.AZFb.AZFc)and SRY for 93 patients including 88 azoospermia and 5 severe oligozoospermia cases by three multiplex PCR technique . 88 azoospermia were divided into two sets: idiopathic azoospermia and non-idiopathic male infertile ,for comparison. Meanwhile testicular biopsy and histological evaluation were performed in 32 azoospermic men .The level of gonadal...
| Keywords/Search Tags: | man infertility, Y chromosome, azoospermia factor, STS-PCR, testicular biopsy, gonadal hormone | PDF Full Text Request | Related items |
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