| Partâ… :Experimental study on viable neutrophils after moderate and severe spinal cord injuries in ratsObjective: To observe dynamic changes of myeloperoxidase(MPO) activity after moderate and severe spinal cord injuries(SCI) in rats, and to study the role of neutrophil in SCI.Method: The models of SCI in rats were established with Allen's method. Rats were divided into three groups: sham operation, moderate SCI, severe SCI. The tissue MPO activity was assessed at 3, 12, 24, 48 hours as well as 1 week after SCI respectively. At post-operative 24 hours and 1 week, the spinal cord function of rats was evaluated using inclined-plane test and BBB(Basso, Beattie and Bresnahan) scales. Meanwhile, the pathological changes were observed.Result: The tissue MPO activity significantly increased in two SCI groups at 3 hours post-operative(P<0.01), reached peak at 24 hours(P<0.01), then dropped down gradually, and returned to normal level at 1 week. Compared with the moderate group, the MPO activity in the severe group was significantly higher(P<0.05). Motor disturbances were markedly attenuated in the moderate SCI animals(P<0.01). The pathological changes in the moderate group were better than those in the severe group.Conclusion: Activated neutrophils play a role in the mechanisms of spinal cord secondary injury, and to some extent the severity of SCI is relative to the number of viable neutrophils.
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