Experimental Study About The Effect Of Tong Xin Luo On Myocardial Fibrosis In Chronic Stage Of Viral Myocarditis Rats

Experimental Study About The Effect Of Tong Xin Luo On Myocardial Fibrosis In Chronic Stage Of Viral Myocarditis Rats Object: To observe the anti-fibrosis effect of Tong xin luo on myocardial fibrosis in chronic stage of viral myocarditis, try to illustrate preliminarily the anti-fibrosis mechanism of Tong xin luo from the aspect of cell factor, and offer important experiment gist to enlarge application of Tong xin luo. Methods: Establish the model of myocardial fibrosis in chronic stage of viral myocarditis by injecting CVB3 into Balb/c rats, divide these rats into model group ,Tong xin luo low dose group ,Tong xin luo medium dose group, Tong xin luo high dose group and set up normal group, after four weeks of giving medicine execute the animals and observe the ultrastructure of myocardial cell with tramission electron microscope, the quantity of myocardial collagen was observed by staining with H.E and Sirus red in supersaturated picric acid solution; the amount of HA in serum was measured with the method of radiative immunity; the quantitative expression of fibrosis factor—TGFx PDGF was measured with the method of immunity histochemistry. Results: Myocardial collagen in model group increased obviously compared with normal group from the results of electron microscope, H.E, Sirus red in supersaturated picric acid solution. Myocardial collagen in every dose group decreased to different degree and there was significant difference in PCV of model group respetively compared with normal group and high dose group (the former p < 0.01, the later p < 0.05). There was significant difference in the amount of HA in serum of model group compared with normal group (p < 0.01), but no significant difference compared with every dose group. TGF, PDGF expressed in low level in normal group but in model group they...