Chemical Studies On The Aerial Parts Of Indigofera Kirilowii

The roots of Indigofera spp have been used as main substitutes for traditional Chinese drug"ShanDouGen"in many provinces of China, and usually called"MuLanShanDouGen". Chemical study of the roots of Indigofera kirilowii, one of the main resources of"MuLanShanDouGen"resulted in the identification of two new 3-nitropropanoyl-D-glucopyranoses, which comprised acryloyl group rarely oberserved in natural products. In order to make the best of the plant resources, we studied the chemical constituents of the ground parts of Indigofera Kirilowii, including the stems and the leaves.Fifteen compounds were isolated and purified from the aqueous alcohol extracts of the stems and leaves of Indigofera Kirilowii by solvent extraction, silica gel column chromatography and re-crystallization. On the basis of modern spectral analysis (1H-NMR, 13C-NMR) and classical chemical methods, the structures of ten compounds were determined. From the stems, seven compounds were identified as 2,3,6-tri-O-(3-nitropropanoyl)-α-D-glucopyranose (IKSE-1), lupeol (IKSE-2),β-sitosterol (IKSE-3, IKSP-3), 2,3,4,6-tetra-O-(3-nitroproponyl)-α-D-glucopyranose (IKSE-5), luteolin-7-O-glucoside (IKSE-6), daucosterol (IKSE-7), 2,6-di-O- (3-nitropropanoyl)-α-D-glucopyranose (IKSE-8); from the leaves, five compounds were identified as kaempferol-3-O-rutinoside (IKL-2), rutin (IKL-3), quercetin-3-O- glucoside (IKL-4), 2,3,6-tri-O-(3-nitropropanoyl)-α-D- glucopyranose (IKLE-2) and daucosterol (IKLE-3, IKL-5). All the compounds were isolated from the title plant for the first time, and compounds IKSE-6 and IKL-4 were obtained from Indigofera for the first time. Compounds IKSE-1, IKSE-5 (IKLE-2), IKSE-8 could be responsible for the toxicity of the roots of I. kirilowii. According to our experiment results, the content of 3-nitropropanoyl-D-glucopyranoses of the ground parts of I. kirilowii is much lower in the stems and especially in the leaves than in the roots.In this thesis, we also summarized the studies on botanical characteristic, chemical components, bioactivities and clinical applications of Indigofera species.