Study On The Partial Chemical Constituents And The Bioactivities Of Marine Algae Grateloupia Turuturu And Caulerpa Racemosa

The chemical constituents and bioactivities of two economic seaweeds, Grateloupia turuturu Yamada and Caulerpa racemosa collected from South China Sea have been studied. The sulfated polysaccharides of G. turuturu have been extracted, isolated and purifyed, and their antiviral activities have been discussed.The anti Herpes simplex virus 1(HSV-1) and Coxsackie virus B₃(CVB₃) effects of the crude extracts and fractions from G. turuturu were demaonstrated by cytopathic effect (CPE) reduction assay and MTT colorimetric assay. Minimal inhibitory concentration (MIC) was used to determine the antibacterial effects of the crude extracts and fractions from G. turuturu against 6 bacteria strains which treated with broth dilution method. As a result, the petroleum benzin fraction and butanol fraction from G. turuturu show potent inhibitory effects on HSV-1, with the 50% inhibited concentrations (IC50) of 15.63 and 7.81μg/mL. The petroleum benzin fraction from G. turuturu has inhibition to Salmonella choleraesu, Staphylococcus aureus and Bacillus subtilis with the concentration of 5mg/mL.The chemical constituents of the petroleum benzin fraction from G. turuturu and butanol fraction from C. racemosa have been studied with the activity screening results. Two chemical compounds were isolated from G. turuturu and the structures were identified as hexadecanoic acid (â… ) and cholest-5-en-3-ol(3 β) (â…¡). Cholest-4-en-6-one , Cholest-4-en-3-one , Cholesta-4,6-dien-3-ol and Cholesta -4,6-dien-3-one were attained and characterized by gas chromatograph mass spectrometer (GC-MS). Compound II shows good antiviral activities against HSV-1 with the IC50 of 7.81μg/mL. Compound I has inhibition to some bacteria strains with the concentration of 2.5mg/mL.Two chemical compounds were isolated from C. racemosa and the structures were identified as caulerpin (â…¢) and (2S)-1, 2-di-O-palmitoyl-3-O-(6-sulpho-α-D-quinovopyranosyl) glycerol (â…£). The antiviral result of compound â…£ shows it had inhibitory effects on HSV-1 with the IC₅₀ of 31.25μg/mL.The fatty acids compositions and elemental composition in both G. turuturu and...