The Research Of Effects Of The Principle Of Restraining The Hyperactivity Of The Liver Yang On MRNA Expression Of L-Type Calcium Channel A1 C Subunit (CaL-a1C) And Plasma Membrane Calcium-ATPase (PMCA) In Vascular Smooth Muscle Cells Of Spontaneously Hype

Objective: To observe the effects of the Principle of Restraining the Hyperactiv -ity of the Liver Yang on blood pressure and on mRNA expression of L-Type Calcium Channel a1 C subunit (CaL-a1C) and plasma membrane calcium-ATPase (PMCA) in vascular smooth muscle cells of spontaneously hypertensive rat (SHR), to probe into the function of concha haliotidis included in Tian ma gou teng decoction which is the classical prescription of Restraining the Hyperactivity of the Liver Yang, to illuminate the mechanism of the Principle of Restraining the Hyperactivity of the Liver Yang to loosen blood vessel, to supply the test foundation toTraditional Chinese Medicine on preventing and treating hypertension.Methods: 12-week-old SHRs were assigned randomly into 5 groups: group A (treated with Tian ma gou teng decoction), group B (treated with Tian ma gou teng decoction subtracted concha haliotidis), group C (treated with Nifedipine), group D (treated with concha haliotidis), group E (treated with Normal Sodium, NS), each group consisting of 9 rats. Before treatment, to measure bacic systolic blood pressure (SBP) and the serum free calcium concentration of every group; during the 4-week-treatment, to measure SBP of all the groups one time every week. After 4-week-treatment, to measure the serum free calcium concentration with atom imbiber spectrum method, to measure mRNA expression of CaL-alC and PMCA1 with RT-PCR half-fix-quantify method.Results: After 4-week-treatment, compare with pre-treatment, SBP is decreased in A group B group and C group, there were significant differences differences (P<0.05), SBP was decreased in D group, there was no significant difference (P>0.05), SBP ascends along with week-age in E group, but there is no significant difference (P>0.05). After 4-week-treatment, there was significant difference in SBP distinction between A group B group C group and E group (P<0.05), there was no significant difference in SBP distinction between D group and E group (P>0.05); There was no significant difference in SBP distinction between A group B group and C group (P>0.05); There was significant difference in SBP distinction between A group and D...