Study On The Effects Of Retinoic Acid On The Configurational Changes And Alteration Of Nuclear Matrix Proteins In Human Osteosarcoma Cell Line MG-63

In this study, the Retinoic Acid (RA) was used to induce the human osteosarcoma MG-63 cells into differention and its effects were investigated by selective extraction-whole mount optic and transmission electron microscopy and techniques of proteomic. The differentially expressed nuclear matrix proteins were analyzed in order to explore the molecular mechanisms in a system level.The results revealed that the osteosarcoma MG-63 cells were induced into terminal differentiation after treated with 1μmol/L RA as the proliferation of MG-63 cells were inhibited, and the cell cycle were arrested in G0/G1 phase. The malignant morphological and ultrastructural characteristics were reversed, while the expression level of oncogene was downregulated, and the expression level of tumor suppressor gene was upregulated. The bone morphological proteins, such as osteocalcin and osteonectin, were highly increased in the cytochemistry and immunocytochemistry assays.While calciums were accumulated on the surface of MG-63 cells And at the same time,the configuration of nuclear matrix-intermediate filament(NM-IF)was altered. Nuclear matrix proteins(NMPs), selectively extracted from MG-63 cells treated with or without RA, were subjected to proteomic analysis.There were 25 of 43differentially expressed spots were identified, including the gene regulate factors, ubiquitination associated proteins, tumor associated gene products and proteins associated with remodeling of cellular configuration.Such as ZNF710,transcripton elongation A, hnRNP A2/B1,UCH interacting protein, SUMO-4,ZYG11B protein,Cyclin fold protein1,clk3,orphan nuclear receptorNR1D2, RAB3D, Rb-binding7, nucleophosmin, prohibitin, vimentin, actin, MHC classⅠantigen,DORA protein, HSP70,etc.Except vimentin,...