| In this study, a polar compound, hexamethylene biacetamide, was used to induce the human osteosarcoma MG-63 cells into terminal differention, and its effects were investigated by cellular biology and immune biology. The differentially expressed nuclear matrix proteins were analyzed by subcellular proteomic methods in order to find out a new way to explore the molecular machnisms of carcinogenesis and malignant phenotypic reversion in a system level.The results revealed that the osteosarcoma MG-63 cells were induced into terminal differentiation after treated with HMBA as the proliferation of MG-63 cells were inhibited, the cell cycle were arrested in G0/G1, the malignant morphological and ultrastructural characteristics were reversed, the configuration of nuclear matrix-intermediate filament was altered, and calciums were accumulated on the surface of MG-63 cells, the bone morphological proteins, such as osteocalcin and osteonectin, were highly increased in the cytochemistry and immunocytochemistry assays. Nuclear matrix proteins, selectively extracted from MG-63 cells treated with or without HMBA, were subjected to proteomic analysis. Sixteen differentially expressed spots were identified, including up-regulated proteins MHC class II antigen, interferon-stimulated gene factor 3α, 8-hydroxy-guanine glycosylase homolog ogg1, vimentin, DKFZp434M2221.1,down-regulated proteins hnRNP A2 / B1, actin, prohibitin, and newly expressed proteins similar to 60S ribosomal protein L21, ST2 protein. The specific nuclear matrix proteins associated with malignant morphology phenotypic reversion, including vimentin, actin, hnRNP A2/B1 and prohibitin, were identified after comparing the results with that from the RA-induced MG-63 cells and HMBA-induced human gastric mucosa MGc80-3 cells, then confirmed by western blot and immunofluorescence analysis. Prohibitin, one of the specific nuclear matrix proteins, was colocalized with oncogene c-fos and c-myc products, as...
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