| Purpose: 1. To investigate the significance of staining with 2,3,5-Triphenyltetrazolium chloride (TTC) to judge the area of coagulation by microwave quickly. 2. To explore the characteristics of water cooled microwave antenna. 3. To investigate the characteristics of and their change under different combinations of the microwave power and coagulation duration, especially under the circumstance of long time with high power. Material and methods: Rabbits undergo hepatic coagulation at 45W for 3 minutes under US-guided percutaneous microwave coagulation, and to observe TTC as well as other results under the light, electron microscope and ultrasound. Porcine liver in vitro/in vivo is coagulated by water cooled microwave antenna at different power and time, and to observe TTC of the porcine liver.Results: 1. The areas of coagulation necrosis can not be stained with TTC, otherwise be red with TTC. 2. The cell morphology of liver stained with TTC can be observed under the light microscope. 3. With enhanced power and prolonged time, water cooled microwave antenna can increase the coagulation as well as carbonization areas of porcine liver in vitro and in vivo. And the speed of increasing is inverse proportion to the time. On the other hand, under the same circumstance, the stronger effects of coagulation can be found in vitro. Without Water cooled equipment the coagulation area of the porcine liver is relatively long, and the column temperature is higher than 27℃to 48℃~60℃.Conclusion: TTC can judge the area coagulated by microwave immediately, simply and truly. The area without TTC is coagulated area, and red area is non-coagulated area. Water cooled microwave antenna make radiation with high power and long time microwave available and increase dramatically the coagulated area of single puncture. The column temperature of water cooled microwave antenna keep constantly during therapy. The modality of the coagulated area is resembled to sphere without a trail.
|
PDF Full Text Request