The Experimental Study Of Man Shen No1 Prescription On α-SMA And TGF-β1 In Mesangial Proliferative Glomerulonephritis Rats

Objective:In this study, model of mesangial proliferative glomerulonephritis (MsPGN) was made with immunization. The MsPGN rats were cured by Man Shen No1 Prescription. Proteinuria, renal function, renal pathomorphology, the expression of renalα-smooth muscle actin(α-SMA) and transforming growth factorβl (TGF-β1) were observed. To make sure the effect Man Shen No1 Prescription, and mechanism of Man Shen No1 Prescription on MsPGN were investigated so as to provid experimental data.Methods:The rats model of mesangial proliferative glomerulonephritis was established by inject BSA and LPS. After four weeks, we selected 20 rats that they were obvious higher than others about the 24h urine protein,blood urea nituogen(BUN) and creatinine(Cr),and divided them into model group and treatment group at random. Model group received no treatment. Treatment group treated with Man Shen No1 Prescription four weeks. At the same time, we selected 10 normal rats as normal group. 24h urine protein was determined with the coomassie brilliant method. Cr was determined with trinitrophenal deproteinization method. BUN was determined with diacetyal oxim method. Each group of the mesangial cells(MCs) and the extracellular matrix (ECM) were observed with light microscope. The expression ofα-SMA and TGF-β1 were observed with the immunohistochemical staining. Immunohistochemistry staining was analyzed with the pathologyimage analysis system.Result: 24h urinary protein quantitative analysis showed that: The excretion of urinary protein in model group was higher than normal group's (P<0.01); In treatment group, urine protein quantitative was descent remarkably, compared with model group and there was remarkable difference (P<0.01). Each group of BUN and Cr analysis showed that: BUN and Cr of the blood serum in model group were higher than normal group (P<0.01); Meanwhile, BUN and Cr in treatment group descented significantly than model group (P<0.01). Nephridial tissue pathomorphology showed that: In model group, the mesangial region expanded obviously, matrix increased, blood capillary narrowed or disappeared and index of mesangial matrix increased; Compared with the model group, the above pathology change of treatment group obviously reduced, matrix index remarkable dropped,blood capillary were obviously improved. In nephridial tissue, immunohistochemistry result ofα-SMA,TGF-β1: Compared with normal group, the expression ofα-SMA,TGF-β1 was obviously enhanced in the model group(P<0.01). Compared with model group, the expression ofα-SMA,TGF-β1 was diminution in treatment group(P<0.01).Conclusions: 1. Man Shen No1 Prescription can significantly reduce MsPGN rat's 24h urinary protein,BUN and Cr, improve the change of glomerular pathology to protect renal function. 2. Man Shen No1 Prescription can inhibit the expression ofα-SMA in nephridial tissue,refrain MsPGN rat's MC proliferation. 3. Man Shen No1 Prescription can inhibit the expression of TGF-β1 in nephridial tissue , reduce ECM deposition and delay process of MsPGN. Possibly it is the mechanism of Man Shen No1 Prescription on MsPGN.