| ObjectiveBased on observing the pathological signs and the dynamic change of cell factor for radiation-induced lung injury of Rat in the model caused by multiple low dose exposure , to evaluate the treatment effect and dose-effect relationships of different doses of the blood-cooling and promoting blood flow drugs on Radiation-induced Lung Injury of Rat, and provide Experimental basis for research and empolder of the new drugs. Methods72 Wistar mice were randomly allocated into the irradiation group(group A), the small-dose drugs group(group B, 9g/kg·d), Middle-dose group(group C,18g/kg·d) and high-dose group(group D, 36g/kg·d). the drugs include Rehannia glutinosa Libosh, Forsythia suspense Vabhl, Ligusticum chuanxiong Hort, Pheretima aspergillum, etc. The mice in group B～D started to be treated with blood-cooling and blood flow-promoting drugs by gastrogavage once daily from 1 week before radiation. Coordinate dosing on the basis of mice' weight change once a week .the mice in the irradiation group were treated with 2ml NS. All Mice in once high dose exposure group were radiated at a dose of 30Gy twice weekly, total 10 times .To observe Mental state, diet, activity ,exudates and defecation . 6 mice in every group were choosed randomly and killed on 10 tims, 12 and 26 week after irradiation in right lung, then observed and investigated the histological change of lungs stained with HE. Measuring the different expression of IL-6,TGF-β, TNF-α, bFGF every groups using Image pro plus5.0 after immunohistochemical stain. Results were processed using CHISS Statistical Software. Through general state, living specimens observation, the pathological signs and the dynamic change of cell factor for Rat .to evaluate the effect of the prevention & treatment on new drugs. Results1.During living specimens observation, compared with drugs groups , the irradiation group presented a symptom of less eat, less activities ,and dry stool; There's no difference between middle-dose group and high-dose group ,however the symptom of petechia around nose and dry stool in group C&D was less than group B.2.The surface of right lung in the irradiation group was swelling and presented Patchy hemorrhage since the fifth week; these symptoms in the 12th week were better than before ;during 26th weeks the lung showed focal interstitial fibrosis which was consistent with the symptom of reduced volume and hard surface. The group B presented lots of petechiae since the fifth week; the surface of right lung began less petechiae; during 26th week, the symptom of reduced volume and hard surface was less than before. Group C&D were less swelling and hemorrhagic; the surface of right lung began petechiae from 12th week ; During 26th week, volume of right lung wasn't reduced ,the the symptom was normal.3.To observe the lung beneath a microscope , the irradiation group in the fifth week presented symptoms of interstitial lung congestion , edema and alveolar structural damage; during 12th week , acute radiation-induced pneumonia was reduced, alveolar interstitial thickening, alveolar spaces smaller and more Lymphocyte infiltration; since 26th week, alveolar interstitial serious thickening, alveolar spaces smaller and disappeared, Fibroblasts increase. With increased drugs, symptoms of interstitial lung congestion , edema and alveolar structural damage in the group B～D were less than before; during 12th week, alveolar interstitial thickening wasn't serious; during 26th week, group C&D presentedlight alveolar structural damage and little Fibroblasts. There was no obvious difference between Group C and D.4.based on the color of the Immunohistochemical staining, TGF-βand TNF-α, IL-6 were expressed obviously in the irradiation group since the fifth week, however TGF-β bFGF were expressed obviously since 12th week and 26th week ;drugs groups can effectively prevent the expression of TNF-α & IL-6, especially in the group C&D; TGF-β(P<0.01)were expressed less than the irradiation group. bFGF were expressed less, but group D can effectively prevent the expression of bFGF since 26th week . There is no different expression with TNF-α,IL-6 and TGF-β(P>0.05) in the group C&D. Conclusions1.Mice presented symptoms of dry stool and petechia around nose swelling and hemorrhagic Surface of Lung tissue in vivo than the irradiation group, were the symptom of "pyretic toxicity accumulate inside" and "heat impairing pulmonary collateral", blood-cooling and blood flow-promoting drugs can prevent and reduce pristine radiation-induced lung injury . during the late term, the symptom of petechiae and focal interstitial fibrosis was better than before, indicated that blood-cooling and blood flow-promoting drugs can reduce the symptom of "stagnant blood hinder pulmonary collateral" in the late radiation-induced lung injury.2. blood-cooling and blood flow-promoting drugs can reduce swelling and hemorrhagic Surface of Lung tissue in vivo, Interstitial lung congestion, edema and alveolar structural damage in the pristine radiation-induced lung injury, the drugs can prevent and reduce the acute radiation-induced pneumonia; and can delay the progress of pulmonary fibrosis in the late radiation-induced lung injury.3. blood-cooling and blood flow-promoting drugs can early prevent the expression of TNF-α & IL-6, especially can effectively prevent the expression of TGF-β& bFGF in the late radiation-induced lung injury. It's the prevention mechanism used to reduce acute pristine radiation-induced pneumonia and delaythe progress of pulmonary fibrosis, therefore drugs can prevent and cure of pristine radiation-induced lung injury. Reduced the progress of pulmonary fibrosis by drugs maybe have relationship with reducing acute pristine radiation-induced pneumonia and preventing pulmonary fibrosis.4. blood-cooling and blood flow-promoting drugs has the effect of prevention and treatment for the early radiation-induced lung injury, Middle-dose and high -doses group were better than small-dose, however there's no difference between them. Suggest that using the adequate doses of blood-cooling and blood flow-promoting drugs would not enhance the effects of prevention and cure for radiation-induced lung injury if the doses of drugs were aggrandized.
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