The Influence Of The Sub-thalamic Nuclei High Frequency Stimulation In Dopamine Metabolism Of Parkinson's Disease Rat Model

In the present study, the effects of bilateral sub-thalamic nuclei (STN) high-frequency stimulation (HFS) in Parkinson's disease (PD) have been investigated using the means of molecular biology, behavioral test and the possible mechanisms involved were discussed. The research includes two parts:1. The rotation rate of PD rat models before and after STN-HFS: A rat model of PD is induced by unilateral administration of 6-OHDA into right substantia nigra and the ventral tegmental area. The rotation rate is measured before and after STN-HFS. Our data suggest that after STN-HFS there is a partial reversal of rotational behavior of rat models.2. The influence of STN-HFS in nigral and striatal TH protein and gene expression: We investigated the effects of STN-HFS on striatal dopamine metabolism using RT-PCR and Western blot of striatal and nigral tyrosine hydroxylase (TH). Our data suggest that STN-HFS enhances TH protein and gene expression in substantial nigra and striatum. TH is the key enzyme for dopamine synthesis, we, therefore, conclude that the application of STN-HFS could compensate for the dopamine deficit by inducing increased striatal dopamine release and metabolism.Conclusion: The present results support that the application of STN-HFS could compensate for the dopamine deficit by modification of either protein or gene expression of the rate-limiting enzyme. The effectiveness of STN-HFS in PD patients may be partially mediated via the striatal DA system. And STN-HFS maybe the most effective method for PD treatment.