The Titania Surface Derived By Treatment Of Ti Substrate With A H₂O₂/HCl Solution And Heat Modulates The Response Of MC3T3-E1 Osteoblast-like Cells

The attachment, proliferation and differentiation of MC3T3-E1 osteoblast-like cells were studied on polished Ti (PT), roughened Ti (SAT) and the amorphous titania gel layer, which was formed by treatment Ti substrates with H₂O₂/HCL solution, coated roughened Ti substrates (SAHT). The surface properties of three kinds of Ti disks were investigated by scanning electron microscope and atomic force microscope. The bioactivity of samples was assessed by evaluating MC3T3-E1 osteoblast-like cells responses by using Fluorescence microscopy, calculating shape factor, MTT tests and real-time RT PCR. The cells attached more quickly on SAHT surfaces; Also, at the first few hours cells on PT surfaces developed an extremely regular cell shape, but on SAT and SAHT surfaces a more irregular shape with more pseudopodia-like structure. The proliferation of cells on all surfaces was no difference just as the MTT test showed. Real-time RT PCR show significant (p<0.05) increases in cbfa-1, AKP-2 and BSP gene expression in osteoblasts grown on SAHT surfaces at 3 d, relative to SAT surfaces. Osteocalcin and collgen type I gene expression were significantly (p<0.05) increased on SAHT surfaces at 7 d compared with SAT surfaces. On the contrary, osterix and BSP level were significant (p=0.000) lower on SAHT surfaces at 7 d. In conclusion that titania gel layer, derived by treatment of Ti with H₂O₂/HCL solution, coated on roughened Ti substrates promote adhesion and influence the differentiation of osteoblastic cells.