Transplantation Of Anoxic Precondition Mesenchymal Stem Cells Treats Post-infarction Ventricular Remodeling In Rats

AIM: This study was performed to investigate the efficiency of anoxic precondition mesenchymal stem cells transplantation(MSCs) on ventricular remodeling and heart function after myocardial infarction in rats and the mechanism of MSCs transplantation. METHODS: MSCs and was acquired from the bone marrow of adult rats. They were isolated, purified and cultured in ex vivo before transplantion. One week after left coronary artery ligation, the cultured cells, marked with BrdU, were injected directly into the border of infarcts. 1 week and 4 weeks after transplantation, Rats' heart function, ventricular remodeling and pathology results were measured by echocardiography, histology and immunohistochemistry. RESULTS: Transplantation of anoxic precondition MSCs was associated with improved left ventricular function, as compared with the control. Transplantation prevented cardiac dilatation and reduced collagen content in myocardium. The numbers of blood vessels and cardiomyocytes were increased at the boundary of infarction site in the animals transplanted with anoxic precondition MSCs. Some cells in transplanted region stained positively for Brdu and so did the endothelial cells of the blood vessels. CONCLUSION: Anoxic precondition MSCs can survive and home in host infarct hearts without addition of any immunosuppressant. MSCs transplantation has benificial effects on remodeling processes and contributes to the improvement of cardiac function, possibily resulting from the reduction of the amount of the collagen, increased myogenesis and angiogenesis And paracrine effect is also suggested.