The Association Among In The Genetic Polymorphisms Of TAP2 And Hepatitis B Virus Infection And HBVsCTL

Objective:To investigate the association among in the genetic polymorphisms of TAP2 and Chinese patients with hepatitis B virus infection and HBcAg specific CD8~+ cells.Methods:1. According to the definition of The Blue Print of Prevention and Cure of Virosis Hepatitis-2000, 110 patients with chronic active hepatitis B, hepatic cirrhosis or chronic asymptomatic HBV carriers were selected.Choose 76 healthy individuals as control group.2. TAP2 gene genotypes were determined by means of PCR-RFLP and HBV was detected by means of ELISA.3. HBVsCTL in whole blood samples of chronic hepatitis B patients were stained with soluble HLA-A2-HBV core 18-27 peptide tetrameric complex were counted by flow cytometry.4. The results were analysisted by SPSS 12.0. The frequency of the genetic polymorphisms of TAP2 among in chronic active hepatitis B, hepatic cirrhosis, chronic asymptomatic HBV carriers and healthy control were compared by x~2 test; The proportions of HBVsCTL in whole blood samples of chronic hepatitis B patients between different TAP2 genetypes were compared by F test. P < 0.05 were significantly.Results: 1. In the HBV-infected patients, frequency of TAP2-651C/C was significantly lower than in healthy control group(P<0.05 ) and TAP2-651R/C was significantly higher than in healthy control group(p<0.05) .2. In chronic active hepatitis B patients, frequency of TAP2-651C/C was significantly lower than in healthy control group (P<0.01) and TAP2-651R/C was significantly higher than in healthy control group (P<0.05).3. In hepatic cirrhosis patients, frequency of TAP2-651C/C was significantly lower than in healthy control group (P<0.01 ) and TAP2-651R/C was significantly higher than in healthy control group (P<0.05).4. In chronic asymptomatic HBV carrier, frequency of TAP2-687Q/Q was significantly lower than in healthy control group (P<0.05) and TAP2-687S/S was significantly higher than in healthy control group (P<0.05).5. In the unmoral ALT group(ALT > 40U/L), frequency of TAP2-651C/C was significantly lower than in normal ALT group (P<0.01 ) ; Frequency of TAP2-687Q/Q was significantly lower than in normal ALT group (P<0.05).6. There was not significantly different in the proportions of HBVsCTL in whole blood samples of chronic hepatitis B patients with three TAP2-651 genetypes (P>0.05) .7. There was not significantly different in the proportions of HBVsCTL in whole blood samples of chronic hepatitis B patients with three TAP2-687 genetypes (P>0.05) .Conclusion:1. TAP2-651C/C gene type might be resistant to chronic active hepatitis B and hepatic cirrhosis.2. TAP2-651R/C gene type might be associated with susceptibility of chronic active hepatitis B and hepatic cirrhosis. 3. TAP2-687S/S gene type might be associated with chronic asymptomatic HBV carrier state.4. The genetic polymorphisms of TAP2-651 and TAP2-687 might be independent with the proportions of HBVsCTL in whole blood samples of chronic hepatitis B patients.