Polymorphisms Of Transporter Associated With Antigen Processing And Hepatitis B Virus Genotypes In Patients With Hepatocellular Carcinoma

Objective: To examine the distribution of transporter associated with antigen processing (TAP) alleles and hepatitis B virus( HBV) genotypes in patients with hepatocellular carcinoma(HCC),then to investigate the association of TAP polymorphism with HCC and HBV .Methods: The polymorphisms of TAP in 76 HCC patients and 150 controls were distinguished by the methods of PCR-ARMS and PCR-RFLP . The HBV genotyping in HBV DNA positive subjects among 76 cases was performed by the method based on nested PCR using type-specific primers .Results: The prevalent frequencies of TAP 1 alleles in HCC patients are TAP] 333 He, TAPi 637 Asp, TAP] 333 lie-lie, TAP] 637 Asp-Asp and TAP1A haplotype(67.8%, 76.3%, 51.3%, 59.2%, 65.0%) . The prevalent frequencies of TAP2 alleles in HCC patients are TAP2379Val , TAP2 565Ala , TAP2651Arg , TAP2665Ala , TAP2 379 Ile-Val , TAP2565Ala-Ala , TAP2651Arg-Arg , TAP2665Ala-Thr , TAP2A haplotype, TAP2B haplotype and TAP2C haplotype(57.9%, 80.9%, 84.9%, 55.9%, 52.6%, 65.8%, 69.7%, 69.7%, 25.0%, 38.6%, 17.0%) . HBV genotyping was performed in 52 HBV DNA positive subjects of HCC patients . The distribution of HBV genotypes is 30 of type B and 22 of type C. There were not other types to be observed. The frequencies of TAP2 665 Ala, TAP2665 Ala-Ala and TAP2B haplotype are significantly increased in patients compared with controls (P < 0.05 OR=1. 56 95%CI=1. 05-2. 30 , P < 0. 05 OR=2. 59 95%CI=1. 19-5. 65, P < 0. 05 OR=1. 80 95%CI=1. 06-3. 04) . The patients' group was further divided into three groups based on patients ' HBVgenotypes . The frequencies of TAP alleles in the three groups did not showsignificantly deference.Conclusion : TAP2 665 Ala, TAP2 665 Ala-Ala and TAP2B haplotype could beassociated with an increased risk of developing hepatocellular carcinoma.