Expression, Transcription And Significance Of VEGF-C In Human Hepatocellular Carcinoma

Objective: to study the expression, the transcription and the character of VEGF-C in human hepatocellular carcinoma, and discuss the role of VEGF-C in the occurrence and development of human hepatocellular carcinoma.Methods: 30 cases of the human hepatocellular carcinoma were examined by immunohistochemical method and RT-PCR for VEGF-C; the 7402 human hepatocellular carcinoma cells were examined by immunohistochemical method. The results of RT-PCR were showed with the relative coefficient of gray scale value of the electriphoresis band.Results: 1. Of the 30 cases, there was 27 cases expressing VEGF-C in HCC tissue and the positive rate was 90.0%, which showed the high expression of VEGF-C in HCC tissue. 2. VEGF-C was expressed in the cytoplasma of HCC tissue and the 7402 human hepatocellular carcinoma cells. 3. The larger the diameter and the lower the differentiation degree of HCC, the higher the expression of VEGF-C was. 4. VEGF-CmRNA was expressed in HCC tissue and nomal hepatic tissue, and the relative coefficient of gray scale value of the electriphoresis band was 233.7% and 78.1%.Conclusion: 1. There was a high expression of VEGF-C in HCC tissue and VEGF-C played an important role in the occurrence and development of human hepatocellular carcinoma, which was maybe related with the quick progress of HCC. 2. Hepatocarcinoma cells also expressed VEGF-C in vitro. 3. The expression of VEGF-C was related with the diameter and differentiation degree of HCC.