The Relationships Between The Expression Of COX-2,VEGF-C And Angiogenesis,Lymphangiogenesis,Prognosis In Rectal Cancer Of Stage Ⅱ And Ⅲ

PART IExpressions of COX-2 and VEGF-C in rectal cancer of stage II and III and the relationships between them and tumor angiogenesis andprognosisObjective To investigate the expressions of Cycloxygenase - 2 (COX-2) and vascular endothelial growth factor (VEGF-C) in rectal cancer of stage II and III, to explore the relationships between them and the tumor biological characteristics, tumor angiogenesis. Methods The expressions of COX-2, VEGF-C and the microvessel density (MVD) were detected by immunohistochemical staining. Results ①The positive rates of COX-2 and VEGF-C expression in rectal cancer were 72.5% , higher than those of peritumoral normal tissue ( P < 0.05). The value of MVD of cancer is 18.41±8.859, higher than the value of peritumoral normal tissue(P< 0.05). ②)The expression of COX-2 was correlated with the expression of VEGF-C, CD44, Neu, and also correlated with the differential of the tumor and the depth of the invasion, it was not correlated with the lymph node metastasis and the size of the tumor. ③The expression of VEGF-C was correlated with the expression of COX-2 only, it was not correlated with the lymph node metastasis , the size of the tumor, the differential of the tumor and the depth of the invasion, ④The value of MVD was correlated with the expression of COX-2, VEGF-C, Neu, and also correlated with the differential of the tumor and it was not correlated with the lymph node metastasis, the size of the tumor and the depth of the invasion. Conclusion The expressions of COX-2 and VEGF-C in rectal cancer of stage II and III may be related to tumorangiogenesis, but have no relationship with prognosis. PART IIExpressions of COX-2 and VEGF-C in rectal cancer of stage II and III and the relationships between them and tumor lymphangiogenesis andprognosisObjective To investigate the expressions of Cycloxygenase - 2 (COX-2) and vascular endothelial growth factor (VEGF-C) in rectal cancer of stage II and III, to explore the relationships between them and the tumor biological characteristics, tumor lymphangiogenesis. Methods The expressions of COX-2, VEGF-C and the lymphatic vessel density (LVD) were detected by immunohistochemical staining. Results ①The value of LVD of cancer is 15.35±5.861, higher than the value of peritumoral normal tissue(P< 0.01). The value of LVD was correlated with the lymph node metastasis and prognosis. ②The value of LVD was not correlated with the expression of COX-2, VEGF-C, it was not correlated with the differential of the tumor, the size of the tumor and the depth of the invasion too. Conclusion The lymphangiogenesis mainly occurs in peritumoral areas, LVD is correlated with the lymph node metastasis and prognosis.PART III The effect of COX-2 selected inhibitor celebrex on the angiogenesis andlymphangiogenesis in the LoVo xenograftObjective: To investigate the effect of celebrex (celecoxib) on human colon carcinoma cell line implanted in nude mice in vivo, to investigate the effect of celebrex on the expression of COX-2 and VEGF-C, to study the effect of celebrex on angiogenesis and lymphangiogenesis. Methods: Thetransplantable human colon carcinoma tumor models in nude mice were established and were divided into 3 groups at random, and treated with placebo, Celebrex 40mg/kg, Celebrex 80mg/kg, respectively. Mice were sacrified at 22th day. Count the tumor inhibition rate and relative tumor proliferation rate. The expressions of COX-2, VEGF-C and the microvessel density (MVD), the lymphatic vessel density (LVD) were detected by immunohistochemical staining. Results: ①the tumor inhibition rate of low-dose group and high-dose group is 33.8%and 28.7%, respectively. (2)relative tumor proliferation rate of low-dose group and high-dose group is 17.9%和16.5%, respectiveiy. (3)the expression of COX-2 and VEGF-C is correlated, but there is no difference among three groups. (4)MVD is correlated with the expression of COX-2 and VEGF-C, the level of MVD in the groups with Celebrex is lower than the contrals.(5)the level of LVD is not correlated with the expression of COX-2 and VEGF-C, there is no difference among three groups. Conclusions: the expression of COX-2 and VEGF-C is correlated with the angiogenesis of the transplant tumor, but has no effect on the lymphangiogenesis; Celebrex can inhibit the angiogenesis of the transplant tumor, but has no effect on the lymphangiogenesis of the transplant tumor.