Effects Of Alcohol On HBV Gene Mutations With Chronic Hepatitis B

Backgroud :Hepatitis B virus (HBV) infection and chronic alcoholism are major public heath problems worldwide. Alcohol have significantly effect on prognosis of hepatitis B. More importantly, habitual alcohol intake has been proposed to be an accelerating factor for the development of hepatocellular carcinoma in chronic hepatitis B. Hepatitis B virus infection is more frequently found in alcoholics than non-alcoholics. Several authors have report that ethanol stimulates HBV DNA replication and gene expression in HBV transgenic mice. HBV infection may be a positive risk factor for alcoholic liver disease. However ,little is still known about the relationship between HBV gene mutation and habitual alcohol intake.Objective: In this paper, we want to study alcohol influence on gene mutations of HBV DNA by detecting serum in alcoholics and non-alcoholics with gene chip ,and to offer testimony for clinical diagnosis and treatment of chronic hepatitis B.Methods: 45 patients with chronic hepatitis B were divided into two groups,20 alcoholics and 25 non-alcoholics, An alcoholic in the present study was defined as an individual who drank alcoholic beverages containing more than 80 g ethanol/day for more than 10 years just prior to admission. Gene chip technique was used to detect gene mutations located in Pre-C nt1896 and nt1814, basal core promoter(BCP) ntl762 and nt1764, P nt528 and nt552.Results: Gender age genetype of HBV, the history of blood transfusion alanine aminotransferase levels and clinical diagnosis of studied patients were not statistically different between alcoholics and non-alcoholics with chronic hepatitis B .The frequency of mutation on BCP nt1762 and nt1764 in alcoholic group are both significantly higher than non-alcoholic group(p<0.05). The frequency of mutation on Pre-c nt1896 nt1814 and P nt528 nt552 have no difference between alcoholic and non-alcoholic group (p>0.05 ) .Conclusion: Alcohol can stimulate HBV gene mutations on BCP nt1762 and nt1764, gene mutations on BCP may enhance HBV DNA replication and gene expression,Leading to aggravate the clinical outcome of hepatitis B virus (HBV)-related chronic liver diseases and to increase the risk of liver cirrhosis, hepatocellular carcinoma. Gene mutations on BCP in alcoholics may be the mechanism of leading to consistent HBV infection replication and gene expression. In this paper ,we found that The frequency of mutation on Pre-c nt1896 nt1814 and P nt528 nt552 have no difference between alcoholic and non-alcoholic group, limited case may be answered for the outcome, or alcohol has no influence on gene mutations of HBV DNA located in Pre-C and P.