Qingdao Region Of Hepatitis B Virus The Bcp Gene Mutation And Hepatitis B Virus Associated With Primary Liver Cancer Research

Objective:Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in the world. There are about 598,000 deaths each year and about 626,000 new cases annually in the world. The death rate is similar to the incidence. Due to the difficulty of diagnosing in time, HCC has a poor prognosis after clinical diagnosis, so, it is important to diagnose and intervent early of primary carcinoma of the liver. Studies have shown that hepatitis B virus (HBV) infection's risks of hepatocellular carcinoma are 100 times higher than nomal population. Currently, many studies have shown that mutations of hepatitis B virus are closely related to HCC, especially hepatitis B basal virus core promoter (BCP) gene T1753V, A1762T/G1764Aalleles double mutantion, are closely related with HCC. We analyzed the Han population's mutations features in hepatitis B virus core promoter of Qingdao, Shandong Province, to explore the relevance of HBV core promoter mutations with primary liver cancer. Methods:Serum specimens of patients with chronic hepatitis B (n=30) and hepatitis B related hepatocellular carcinoma (n=30) were collected in this study, then, extracted HBV DNA. Using polymerase chain reaction (PCR) amplific -cated S gene fragments to determine patients' genotype and serotype according to its coding sequence; amplifying HBV DNA core promoter gene and cloned, sequencing. To analyze the mutations used DNAMAN software by comparing the results with the standard strains of HBV in the GeneBank. SPSS 17.0 software was used for statistical analysis. Rusults:All the samples (n=60) have amplified S gene. All the HBV strains could be categorized into genotype B or C, of which the major gnentype is C-type, and serotypes adw2 or adrq+. Except 2 patients with chronic hepatitis B patients and 7 cases with liver cancer patients, all the rest have amplified 619bp gene fragment, which contain the core promoter. In HBV core promoter region, the common point mutations were T1753V, C1653T and A1762T/G1764A, which rates were 86.27%(44/51),41.18%(21/51) and 45.12%(23/51), respectively. Compared with patients with chronic hepatitis B, point mutations C1653T (P<.001) and A1762T/G1764A (P<.001), patients with liver cancer had a higher incidence. Conclusion: The HBV genome common genetypes were B and C, of which the major gnentype is C-type; There is a high incidence of mutations in BCP gene, such as T1753 V may have nothing to do with liver cancer and C1653T, A1762T/G1764A alleles double mutantion might have an association with HCC in Qingdao, Shandong Province.