Animal Experimental Study And Development Of Equipment For Osteoporosis Treatment Based On High-Frequency, Low-Magnitude Vibration

Osteoporosis (OP) is a senile orthopedic disease, and it is characterized by easy occurrence and hard cure, harmful for human health. With the population explosion, China has entered the ageing society, and the OP has received more and more attention. Currently, the primary methods for curing OP are drug therapy, nutrition therapy, and exercise therapy, or the combination of the three therapies. Faced with the problems, such as the drug-resistance, gastrointestinal stimulation there is a high need to develop a non-drug, noninvasive therapy for curing OP. In the past research, when the vibration worked on the human body, the bone's mechanical structure would be changed, the array of trabecular would be changed, and these changes would stimulate the bone to grow, so the vibration could anti OP, and some researchers reported that the vibration within a certain range can stimulate bone growth, and they made the animal experiment research on vibration accordingly.Based on the domestic literature and the animal loading experiment of our research group, the vibration range was determined, the vibration within the determined may be beneficial for of the OP suffer. Three possible blue prints were put forth, but only the machinery vibration was designed and conducted, through the f = 32Hz, a = 0.3g vibration on the rats, for 8 weeks.For the multiple primary OP, the ovariectomized rats models (simulated the I type OP ) and the tail-suspension rats models (simulated theâ…¡type OP ) were established, through the f = 32Hz, a = 0.3g vibration on the rats. From the 8-week experiment, the following observations were obtained:(1) the 8 weeks, the ovariectomized group (OVX) and the vibrated ovariectomized group (OVX+V) weighted higher than the other groups, the difference in weight was very significant. The significant difference suggested that it was the ovariectomy surgery that put their weight up rather than the vibration.(2) Cut the tibia of rats to three parts, the middle one was made to a 5mm-high cylinder, as the compact bone specimen, and the spongy bone of approximate tibia was abraded to a 3mm-high cylinder, as the spongy bone specimen. The two specimen were compressed in axial, to measure the elastic modulus (E). It was discovered that the mechanical capability of the vibrated groups were higher in multi-degree. Compared with the OVX, the E of the OVX+V was heightened, and the heightenness was the maximal. So we can get, through the 8-week vibration, the anti-compression ability of rat tibia was heightened, in other words, the quality of the tibia got improved.(3) The horniness slice of tibia of all groups was made; the change in percentage of trabecular area (%Tb.Ar), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) were observed. After 8-week vibration, for the group which received vibration, the number of the %Tb.Ar, the Tb.Th and the Tb.N were heightened, but the number of the Tb.Sp was decreased. These means that the area of trabecular was larger, the thickness of trabecular was stronger, the number of trabecular was increased, and the structure of trabecular was tighter. In the other words, the structure of trabecular of the vibrated groups was better than the other groups, from fine and microcosmic structure.(4) By means of the Micro-CT, not only the microcosmic structure of bone can be observed straightly, but also the quantify analysis can be conducted on the bone . For the comparison between the Micro-CT and the horniness slice, we also analyzed the %Tb.Ar, the Tb.Th, the Tb.N and the Tb.Sp, the trends of the two analyses were basically consistent. But the numerical values got from the Micro-CT were higher than those from the horniness slice in the Tb.Th on the average. And the Tb.Sp of the Micro-CT was positively correlated with the volume of trabecular.The difference between the values got from the Micro-CT and those got from the horniness slice, the Micro-CT was considered to be more precise. Because the operation process of the Micro-CT was straighter, this led to the smaller deviation. And the 3D quantity analy of the Micro-CT was more actual.The measurement of the bone's mechanical properties, horniness slice and Micro-CT showed the elastic moduluses (E) of the compact bone and the spongy bone in the tibia were heightened by the f = 32Hz, a = 0.3g vibration; the area , the thickness, and the number of trabecular were heightened; the structure of trabecular was tighter, over the 8 weeks. That means the mechanical properties of the bone were heightened, and the fine and microcosmic structure of the bone were enhanced by the vibration, in other words, the f = 32Hz, a = 0.3g vibration can be conducive to the...