A Study On The Influence Of The Nano-scaled Hydroxyapatite-sol On The Anaerobic Capacity Of Mice Via Intravenous Injection

1. ObjectiveThrough the experiment on mice ,observing the influence of the nano-scaled hydroxyapatite-sol on the anaerobic capacity of mice via intravenous injection, discuss the mechanism of nano-scaled hydroxyapatite-sol's effects to the anaerobic capacity of mice which will supply with experimental basis and lay theoretical foundation on nano-scaled hydroxyapatite-sol efficacy analysis as well as its exploitation in athletic sports.2. Participants and methodsWe have 42 female KM mice of 12 weeks old, 29³3g weight, bought form the animal scientific research center of Hubei province. The mice were randomly divided into four groups: nano-scaled hydroxyapatite injection group (H group) ,12 samples included; NaHCO₃ injection group (N group) ,10 samples included; distilled water injection group (D group) with 10 samples in it and control group (C group) which also have 10 samples.Take a few days of swimming training to let the mice adapt to swim. The mice in the four groups burden 10% of its weight to take part in the exhausting anaerobic swim test after being injected the corresponding drugs. Record the exhausting time of each mice, measure the blood lactate, the lactate and the glycogen in the skeletal muscle and cardiac muscle, then all data are proceeded statistical analysis by spssl3.0.3. Results3.1 Compare to the mice in D group and C group, both the nano-scaled hydroxyapatite-sol and the NaHCO₃ via intravenous injection can significantly prolong the exhausting time, however, inject the nano-scaled hydroxyapatite-sol has a better effect on prolonging the exhausting time than the NaHCO₃ (P<0.01) .3.2 Compare to the mice in N, D, C groups, the nano-scaled hydroxyapatite-sol via intravenous injection has no significant effect on the blood lactate after the exhausting anaerobic swim test (P>0.05) . 3.3 The lactate in the skeletal muscle of the mice in the H group are significantly lower than in the N, D, C groups (P<0.01); However, the lactate in the skeletal muscle of the mice in the N group didn't show significant difference compare to the D and C group (P>0.05) .3.4 The lactate in the cardiac muscle of the mice in the H group are significantly lower than in the N, D, C groups (P<0.01) ; However, the lactate in the cardiac muscle of the mice in the N group didn't show significant difference compare to the D and C group (P>0.05) .3.5 The glycogen in the skeletal muscle of the mice in the H group are significantly lower than in the D and C groups (P<0.05); However, the glycogen in the skeletal muscle of the mice in the H group didn't show significant difference compare to the N group (P> 0.05) .3.6 The glycogen in the cardiac muscle of the mice in the H group are significantly lower than in the D and C groups (P<0.05) ; However, the glycogen in the cardiac muscle of the mice in the H group didn't show significant difference compare to the N group (P> 0.05) .4. Conclusions4.1 The nano-scaled hydroxyapatite-sol via intravenous injection can significantly improve the anaerobic capacity of mice, and its effect is remarkably higher than the 5% NaHCO₃.4.2 The nano-scaled hydroxyapatite-sol via intravenous injection mainly take effect through neutralizing the lactate which produced by the skeletal muscle and cardiac muscle when taking high intense exercise, preventing the lactate accumulated in the skeletal muscle and cardiac muscle.