The Expression Of P53, Bcl2, CyclinD1 In Large Intestine Carcinoma

It is one important cause of canceration that cell cycle is out of control . In the course of cell cycle , cyclin D1 as a positive regulatory factor can promote the accomplishment of cell cycle . p53 is a negative regulatory factor , which can inhibit proliferation by blocking the key point of cell cycle . Bcl2 is the first gene found for inhibiting apoptosis caused by various reasons . The research in recent years manifests that p53,cyclin D1 and bcl2 not only play important roles in the process of carcinoma of large intestine , but also have effect on histological differentiation degree ,infiltrating degree and metastasis of lymphoid nodes . At present the detection of three genes for early diagnose of carcinoma,estimating the prognosis of carcinoma and gene therapy provide new way for clinical jobs . This research uses immunohistochemical SABC method , the P53 ,bcl2 and cyclin D1 proteins were detected . And we analyze them with patho -diff erentiation degree and clinical stages . The result manifests that the expression of p53,cyclin D1 and Bcl2 protein in carcinoma of large intestine is higher thannormal large intestine tissue . (The normal control group has no expression ) . The expression rates of p53, cyclin D1and bcl2 are 67.5% , 42.5% and 52.5% respectingly in 40 cases of large intestine carcinoma. p53 , cyclinD1 have no expression in 10 cases of normal large intestine tissue , the expression of bcl2 is 10% . The expression of p53 , cyclin D1and bcl2 in carcinoma of large intestine have obvious dependablity with patho -differentiation degree and clinical stage . The expression of p53 is 25% in well differentiated group , 75% in moderately differentiated group , 80% in poorly differentiated group. They have significant differences calculated by statistics . The expression of p53 in DucksC + D group(80.5%) is higher than it is in DucksA + B group(50%) , significant differences between each two groups of them calculated by statistics . The expression of cyclin D1 is 12.5% in well differentiated group , 25% in moderately differentiated group , 65% in poorly differentiated group . Significant differences exist in well differentiated group and poorly differentiated group . So does it between moderately differentiated group and poorly differentiated group . The expression are 25% and 60% in DucksA + B andDucksC + D, significant differences between them calculated by statistics . They have significant differences calculated by statistics . It indicates that cyclin D1 takes an important role in the development of large intestine carcinoma . The expressions of Bcl2 are 25% , 41.7% , 70% in three differentiated groups . Significant differences exist in well differentiated group and poorly differentiated group . The expression are 25 % and80 % in DucksA + B and DucksC + D groups, significant differences between them calculated by statistics . The result sugests that p53 , cyclinD1and bcl2 can be used to evaluate the development and prognostic of large intestine carcinoma . Also it can settle a base for gene detection and gene therapy . The Conclusion suggests that p53 , cyclin D1 and bcl2 take an important role in the development of large intestine carcinoma . It is helpful to judge the infiltrating degree and lymphonode metastasis of large intestine carcinoma .