Study On The Extraction And Separation About The Effective Ingredient Of The Flemingia Philippinensis's And Its Prevention And Treatment With Thrombosis

Flemingia macrophylla is Leguminous plants of Flemingia Roxb . ex Benth for the extraction with the root of Flemingia philippinensis Merr.Encroached on the first experimental study of the extraction with the active ingredient extraction, isolation and identification ; its antithrombotic and experimental study of rats. Flemingia philippinensis for in-depth study and further development of its resources to provide the scientific basis for the benefit of mankind.一,Extraction of Flemingia philippinensis:To extract yield and the total flavonoid content of the comprehensive evaluation index, traditional herbal extract alcohol precipitation method and the method of combining alcohol formulation of effective Flemingia philippinensis component extraction, Extraction of both extract from the TLC analysis to prove that water extraction and alcohol precipitation than single reference to alcohol, So ultimately results by orthogonal be Flemingia philippinensis Merr. water extraction and alcohol precipitation in the best extraction process as 1 : 30 times stood at 75°C water extract 3h, raised a total of three times.二,Separation and identification of components which Flemingia philippinensis:Encroached extraction with aqueous extract alcohol from the extract two compounds were isolated and identified asⅠandⅣ. By TLC and HPLC analysis with the standard under the same conditions, to prove that compoundⅠisβ– sitosterol,CompoundⅣis genistein.三,Flemingia philippinensis extract on the prevention and treament of thrombosis:The experiment used acupuncture rats neointimal formation of thrombus in the model law means thereby avoiding other methods complicated surgical operation. By measuring blood rheology corresponding indicators confirm this method is easy, and the success rate was 100%. Thus setting out for the first time in the extraction with the prevention and treatment of thrombosis. For now Flemingia philippinensis yet to see in the prevention and treatment of thrombosis research reports. Therefore, the results of this study in order to further promote the development of new drugs share the field with a valuable theoretical basis extraction with the in-depth development of the use of certain ingredients in the world.