Studies On The Chemical Constituents And Anti-tumor Activities Of Yao Medicine Cissus Pteroclada Hayata And Flemingia Philippinensis Merr.et Rolfe.

The chemical constituents of Yao medicine Cissus Pteroclada Hayata were studied more systematically in this paper. Nine compounds and one mixture were isolated and identified to be the mixture ofβ-sitosterol, stigmasterol and campesterol(1), beta-amyrin(2), betulinic acid(3),β-sitosterol(4) friedelin(5),11-O-loylbergenin(6),11-O-(4'-hydroxy benzoyl) bergenin(7), daucosterol(8), gallic acid(9), bergenin(10). All the compounds had never been separated from this plant before except the compound 10. Compounds 6 and 7 were obtained from the plants of Cissus for the first time.Volatile oils were exacted by steam distillation(SD) method and ultrasound-assist-ted steam distillation(UESD) method from Yao medicine Cissus Pteroclada Hayata. The results showed that:22 volatile components were identified with the mothod of SD, which accounted for 83.41% of total essential oil.28 volatile components were identified with the mothod of UESD, which accounted for 77.73% of total essential oil. Compared two methods, the yield of volatile oil extracted by UESD was 2 times with its by SD.Five compounds and one mixture were isolated from Flemingia philippinensis Merr.et Rolfe and they were identified to be eriosematin(11), lupeol(12), genistein(13), the mixture of psedo-baptigenin and formononetin(14),3'-O-Methylorobol(15), 5,7,3',4',5'-pentamethoxyflavone(16). The compounds of 15,16 and mixture 14 were isolated from this plant for the first time.The anti-tumor activity of extract in Cissus Pteroclada Hayata and some compounds were studied by MTT method. The results showed that:ethyl acetate extract and butanol extract of Cissus Pteroclada Hayata together with compounds of 6, 7,11,13,15 and mixture 14 had some anti-tumor activity. But other compounds did not have anti-tumor activity. Ethyl acetate extract of Cissus Pteroclada Hayata had better activity in the inhibition of CNE and Hela cells. The IC50 values reached to 35.55μg/mL and 39.74μg/mL.11-O-(4'-hydroxy benzoyl) bergenin and 11-O-galloyl-bergenin showed apparent effect on the growth of cancer cells HT-29, its IC50 values reached to 12.36μg/mL and 16.33μg/mL. Eriosematin inhibitting the growth of cervical cancer Hela was outstanding, its IC50 value reached to 12.22μg/mL. And with increasing concentrations of Eriosematin, inhibition of tumor cells gradually increased.