The Influence Of CpG-ODN On The Immune Effects Of Hepatitis B(HB) DNAvaccine And Recombinant Hepatitis B Vaccine.

Objective To study the influence of CpG-ODNon the immune effects of hepatitis B(HB) DNAvaccine and recombinant hepatitis B vaccine.Methods Divide 30 BALB/c mice into 3 groups,10 for each,and immunize with CpG-ODN+HB DNAvaccine,HB DNA vaccine (positive control) and plasmid pVAX1(negative control) respectively.Divide another 48 BALB/c mice into 6 groups,8 for each.The mice in 4 test groups were immunized with CpG-ODN combined with recombinantHB vaccine at different dosages respectively,using recombi-nant HB vaccine as positive control and physiological saline as negative control.The anti-HBs levels in sera of mice were detected by ELISA. Results Compared with those of positive control,the anti-HBs positive conversion rate of CpG-ODN+HB DNA vaccine group increased by 3 folds,and anti-HBs level increased by 2 folds.Compared with those of CpG-ODN+HB DNAvaccine group,the anti-HBs of the four CpG-ODN+recombinant HB vaccine groups converted early,and the anti-HBs level and positive conversion rate induced by recombinant HB vaccine, even at one-third of the original dosage,showed no significant change.Conclusion CpG-ODN improved the immune effects of both HB DNA and recombinant HB vaccines.