Research On Efficacy And Experimental Ldentification Method For Recombinant Hepatitis B Vaccine (Cell Of CHO)

Objective: Establish ( 2005 version) the effect examination of B a hepatitis of the provision reorganization vaccine( cell of CHO) and discriminate the experiment adoption body outside effect examination method. The method of reorganizing vaccine( cell of CHO) outside the body effect examination have already written into the Medical Dictionary currently, this makes possible to check the effect of reorganized hepatitis of B vaccine using the method of outside the body experiment, also it is necessary. Method: Make a gum of the aluminum body fuse under the condition of acidity, join the Triton-100 to make antigen leave from a solution. Joining to protect can make sample been in accordance with the reference the article condition, and resist the original solution to leave to promote the function. Take the 1 ml adds 100μls of the 1 mol/ L hydrochloric acid,37℃waters for 5 minutes, taking 200μls to add 20μls of 10% Triton,37℃waters for 60 minutes, using the ELisA method examination B liver virus surface antigen( HBsAg), calculate it in the parallel lines method. Result: After many times experiment, establish a hepatitis vaccine, crazy dog vaccine of the reorganization B that oxidize the aluminum and did not handle with the hydrogen and bleed the hot vaccine at the same time, and try a yin of box, the masculine gender conduct and actions to check against. Crazy dog vaccine of result, bleed the hot vaccine and hydrogens to oxidize the aluminum to check against for the feminine gender, the reorganization B a hepatitis of direct examination vaccine( cell of CHO) presents the weak masculine gender, joining the hydrochloric acid to add 10% Triton to present the strong masculine gender after regulate the PH value. Adopt that method examines 5 batches of vaccines to all present the strong masculine gender. The parallelism inside the set experiment, the an experiments again. After repeated experiments, its finished product the solution leaves the coefficient about 1.0, and it is stabilize the reliable. Conclusion: The method can take the place of the small rat experiment conduct and actions reorganization B a hepatitis vaccine( cell of CHO) production line to experiment.