Study On The Extraction Of Total Flavones In The Flower Of Sophora Japonica By The High Pressure Extraction Technology And Its Anti-sunburn Activity

The flower of Sophora japonica was chosen as the raw material. High hydrostatic pressure extraction technology was employed to extract the efficient components from flowers of Sophora japonica. Bioactivityr of the flowers extract was also study.Sophora japonica is one of common and inexpensive Chinese herbal medicine. It has a broader distribution in our country. The flower of Sophora japonica has many health protection functions and has been used as hemostatic medicament. Simultaneously, the flower of Sophora japonica is a good antioxidant, including multiplicate flavone in it. Thus more and more researchers pay more attention to Sophora japonica and its extract.Alkaline water extraction and ethanol reflux are the common methods of total flavones from the flower of Sophora japonica. But these two technologies have disadvantages, such as difficult to separate the active ingredient from the alkaline water, time-consuming and laborious.The ultrahigh isostatic hydrostatic pressure extraction (UHPE) is a new extraction technique. It is to put the extractible matter in the high-pressure container and then extract compound by pressure(100Mpa-1000MPa) under the room temperature. The course can be accomplished in several minutes, it can not only save time but also save strength. Uppermost, the compound extracted by UHPE keeps a preferable stability and bioactivity.The key experimentations in the dissertation are as follows:1. Applied UHPE technology to extract flavones from the flower of Sophora japonica. Seeing about the factors that influenced the extraction of flavone from Sophora japonica by UHPE, such as extraction solvent, ethanol concentration, extraction pressure, extraction times and so on. Finally, the optimum extraction conditions of flavones from the flower of Sophora japonica by UHPE are as follows: 400MPa of extraction pressure; 2min of extraction time;, 80:1 of liquid / Solid ratio; 70% ethanol of extractive solvent; 60min of soak time; once of extraction times. The yield of total flavones is 10.14%. The recursive equation gained by data processing software is: Y=3.44875000+0.3900000000X1+1.3080000000X2+0.08550000000X3+ 0.03200000000X4+0.13150000000X5Correlative quotiety: R= 0.93262, F value= 13.3588,distinct level :p=0.0004.We can conclude that the uppermost factor influencing the process of extraction is extraction solvent, next is pressure. Alternant effect is inapparent.2. To compare UHPE with other four extraction methods.The extraction yields of total flavones are 10.69%,10.12%,8.54% and 12.76% of alcohol reflux, Ultrasonic extraction, decoction extraction and soxhlet method. The extraction time of UHPE is shorter than other foure methods. Thereby, UHPE is the optimal extraction method for the extraction of flavones from the flower of Sophora japonica.3. Measuring the antioxidant activity of the extracts of alcohol reflux, Ultrasonic extraction, decoction extraction, soxhlet method and UHPE by DPPH. The results show that theextract of UHPE has the highest antioxidant activity. The antioxidant rate is over 80%.4. Based on the extraction processing, we also used the macroporous adsorption resin to purify the flavone extracted from the flower of Sophora japonica. The optimum conditions of separating the flavone in Sophora japonica by D101 macroporous adsorption resin are as follows: the speed of adsorption and desorption ,1.5BV/h; concentration of desorption,80%; dosage of water,15BV; dosage of ethanol,4BV; the total flavones content achieve 58.82% after purified. Dissolving the extract purified with petroleum ether, and heating up it, it can remove the pigments and other impurities from the extracts and gained more purified flavones.5. Studied the anti-sunburn activity of the flavones in Sophora japonica through testing the penetrable efficiency of light. We can obviously conclude that the anti-sunburn activity of cosmetic intermingled with flavones is better than the cosmetic without the flavones. The flavonse in Sophora japonica have an obvious anti-sunburn capability. Compared with the anti-sunburn cosmetic in the market, the flavones in Sophora japonica has better anti-sunburn capability, especially in the absorbability of the UVA.