| Loniceraeonfusa is called as Lonicera macranthoides Hand or Lonicera hypolauca Miq or the dry flower or early with open flower of Lonicera confuse.As to be the traditional Chinese medicine that is commonly used in the clinical trials,it shows that it has too many functions,such as anti-inflammatory,antipyretic backdrop,protecting liver,homeostatic,reducing blood fat,oxidation,immune adjustment and so on.As one of the main active constituents in the Lonicera confuse DC,caffeotannic acid is one of the key index in the quality control,which has the functions of antioxidant,anti-free radical,anti-virus and so forth.At present,polyphenols such as chlorogenic acid which was called as "The seventh such nutrients" was widely used in medicine,health,food and cosmetic industries.Therefore,it has a wide range of development and utilization prospects.The price of Chlorogenic acid in high purity is very expensive.Thus,Chlorogenic acid is now internationally recognized as"plant of gold".German scientist Michael did lots of researches on chlorogenic acid in the chemical synthesis method.Under laboratory conditions,Quinic acid by four-step reaction can be of chlorogenic acid.The total output rate is to 65 percent.However,there is no commercial production of chlorogenic acid in the chemical synthesis method.From an economic point of view,Low most of the plants made easy extraction of chlorogenic acid,so the main sources of chlorogenic acid is extracted from natural plants.Chlorogenic acids are organic compounds containing p and o of the phenolic hydroxyl group.Under alkaline conditions,Chlorogenic acids will become susceptible to oxidation and unstable.For this reason,extraction and purification of chlorogenic acid is different from the conventional organic acid extraction.Chlorogenic acid’s extraction method is commonly used on industrial water extracting and alcohol precipitation.But the large amount of alcohol consumption,high costs,lengthy and serious losses of active ingredients after alcohol precipitation allows chlorogenic acid to have lower rates,while impurities in crude extracts,so the losses are large.For current Silver Flower problem of inadequate water extracting and alcohol precipitation process,On the basis of this subject in literature research,we use characteristics of chlorogenic acid-soluble in water,and use water as solvent extraction,separation and purification to reduce the consumption of alcohol in the production process.We have established a green,low mountain of Silver Flower extraction and purification of chlorogenic acid.Around this goal,to study this topic in the following areas:1.completing the method of determing quantities of total organic acids(at chlorogenic acid parity)and chlorogenic acid Lonicera collected from Longhui County,Hunan Province Matang Hill.After verification,Wang Xiaoming-the expert from Hunan Province Academy of Forestry Sciences of shall make a conclusion.Lonicera belongs to Lonicera macranthoides Hand.-Mazz.Recognize 324nm is a detection wavelength.Take chlorogenic acid as control article,adopt ultraviolet spectrophotometry to determine quantities of total orgarnic acids.The results show that,The content of total organic acids(chlorogenic acid)in Lonicera is 108mg/g.Take chlorogenic acid as control article to determine content of chlorogenic acids by High Performance Liquid Chromatography.The results show that,The content of total organic acids(chlorogenic acid)in Lonicera is 60mg/g.2.researching water extraction techniques of total organic acids.By the single factor experimentation,hough indicators like chlorogenic acids extraction percentage、contents of total organic acids to pick out the best abstraction artwork:material ratio is 1:10,abstraction temperature is 50℃C;abstraction time is three,and four hours each time.3.Optimating the processes of removal to Protein and annin in the water extractionSelect ZTC+1-Ⅱ flocculants other four different flocculants,such as the object of review.Investigate single factor experiment to the Screened ZTC+1-Ⅱ.By the crosscut experiment,prioritize the best techniques of removal process in the water extraction:material ratio is 1:10,to join the B component of ZTC +1-Ⅱ B component:A component of the amount of 0.2ml/g(Volume of flocculant solution:the amount of crude drug);Stir for five minutes,stirring temperature is 30℃;stirring speed is 10r/min;adding A component 0.1ml/g;aside for 1 hour.4.studying the methods of removal to fat-soiuble components in the water extraction.Using protein-water extract,investigate AB-8 four kinds of resin adsorption performance of chlorogenic acid and other substances.Results showed that AB-8 can be used for enrichment and purification of chlorogenic acid in Silver flower.The best methods is:Flocculant liquid(1:10);flow rate is 2BV/h;_saturated extent of adsorption is 138.75mg/g(dry resin);Elution of distilled water consumption is 12ml/g(dry resin);Combined outflow of liquid resin and wash off liquid.5.Silver Flower study on removal of carbohydrate composition in water extract.By polyamide adsorption of hydrogen-bonded principle,Chlorogenic acid and sugar components can be separated by polyamides.Technology are as follows:Enrichment process of macroporous adsorption resin Hill Silver Flower solution concentration to density(ρ = 1.04),according to saturated adsorption = 121.29 mg/g(dry polyamide)polyamide column,with 5mL/g(dry polyamide)water elution and collect water eluent.6.Chlorogenic acid crystallization processTake the step of removing the sugar water on aqueous solution,concentrated extracts of such solution,methanol heated to dissolve,and cold on the Crystal,once recrystallization.Finally chlorogenic acid crystals,using the HPLC determination of purity. |
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